绿茶多酚抑制晚期糖基化终产物诱导的大鼠血管平滑肌细胞增殖。
Green tea polyphenols inhibit advanced glycation end product-induced rat vascular smooth muscle cell proliferation.
作者信息
Ouyang Ping, Peng Wen-lie, Xu Ding-li, Lai Wen-yan, Xu An-long
机构信息
Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2004 Mar;24(3):247-51.
OBJECTIVE
To determine the effects of green tea polyphenols(GTP) on advanced glycation end products (AGEs)-induced proliferation and expression of p44/42 mitogen-activated protein kinase (MAPK) of rat vascular smooth muscle cells (VSMCs)
METHODS
Rat aortic VSMCs isolated and cultured in vitro were stimulated with AGEs in the presence or absence of GTP at different concentrations, followed by quantitative analysis of the cell proliferation with colorimetric assay. The p44/42 MAPK activity was evaluated by immunoblotting technique using anti-p44/42 phospho-MAPK antibody.
RESULTS
Compared with the control cells(without GTP treatment), GTP dose-dependently inhibited AGE-stimulated VSMC proliferation (P<0.05), and the p44/42 MAPK activity was significantly enhanced. The effects of AGEs were antagonized by GTP (372+/-41 vs 761+/-56, P<0.05).
CONCLUSION
GTP can inhibit the AGE-induced proliferation and p44/42 MAPK expression of rat VSMCs.
目的
确定绿茶多酚（GTP）对晚期糖基化终末产物（AGEs）诱导的大鼠血管平滑肌细胞（VSMC）增殖及p44/42丝裂原活化蛋白激酶（MAPK）表达的影响。
方法
体外分离培养大鼠主动脉VSMC，在有或无不同浓度GTP存在的情况下用AGEs刺激，然后用比色法对细胞增殖进行定量分析。使用抗p44/42磷酸化MAPK抗体通过免疫印迹技术评估p44/42 MAPK活性。
结果
与对照细胞（未用GTP处理）相比，GTP呈剂量依赖性抑制AGE刺激的VSMC增殖（P<0.05），且p44/42 MAPK活性显著增强。GTP拮抗了AGEs的作用（372±41对761±56，P<0.05）。
结论
GTP可抑制AGE诱导的大鼠VSMC增殖及p44/42 MAPK表达。
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