Dunn-Albanese Lisa R, Ackerman William E, Xie Yubing, Iams Jay D, Kniss Douglas A
Department of Obstetrics and Gynecology, Laboratory of Perinatal Research and Division of Maternal-Fetal Medicine, The Ohio State University, College of Medicine and Public Health, Columbus, Ohio, USA.
Am J Obstet Gynecol. 2004 Mar;190(3):809-16. doi: 10.1016/j.ajog.2003.09.052.
The objective of this study was to test the hypothesis that peroxisome proliferator activated receptor-gamma (PPAR-gamma) is expressed in intrauterine tissues before active term human parturition, and that its repression is associated with up-regulation of cyclooxygenase-2 (COX-2).
Specimens were collected from women with term singleton pregnancies after spontaneous labor or cesarean section before labor, prepared for immunoblot and immunohistochemical analysis, and probed for PPAR-gamma or COX-2.
PPAR-gamma expression was prominent in fetal membranes and placenta before active labor. After labor, PPAR-gamma expression was significantly reduced in fetal membranes, but not in placenta. The ratio of COX-2:PPAR-gamma was significantly elevated in fetal membranes with labor. PPAR-gamma immunostaining was prominent in syncytiotrophoblast, extravillous cytotrophoblasts, and cells of the amnion and chorion. COX-2 immunostaining was abundant in the amnion and rare in the placenta.
PPAR-gamma is highly expressed in term intrauterine tissues. In fetal membranes, PPAR-gamma levels are reduced once active labor commences, coincidental with a relative increase in COX-2 expression.
本研究的目的是验证以下假设，即过氧化物酶体增殖物激活受体γ（PPAR-γ）在人类足月分娩发动前的子宫内组织中表达，且其表达受抑制与环氧合酶-2（COX-2）的上调有关。
收集足月单胎妊娠妇女在自然分娩或剖宫产术前的标本，进行免疫印迹和免疫组织化学分析，检测PPAR-γ或COX-2。
分娩发动前，PPAR-γ在胎膜和胎盘中表达显著。分娩后，PPAR-γ在胎膜中的表达显著降低，但在胎盘中未降低。分娩时胎膜中COX-2:PPAR-γ的比值显著升高。PPAR-γ免疫染色在合体滋养层、绒毛外细胞滋养层以及羊膜和绒毛膜细胞中显著。COX-2免疫染色在羊膜中丰富，在胎盘中少见。
PPAR-γ在足月子宫内组织中高表达。在胎膜中，一旦分娩发动，PPAR-γ水平降低，同时COX-2表达相对增加。
