过氧化物酶体增殖物激活受体γ与环氧化酶-2在人类足月分娩中的相互表达

Reciprocal expression of peroxisome proliferator-activated receptor-gamma and cyclooxygenase-2 in human term parturition.

作者信息

Dunn-Albanese Lisa R, Ackerman William E, Xie Yubing, Iams Jay D, Kniss Douglas A

机构信息

Department of Obstetrics and Gynecology, Laboratory of Perinatal Research and Division of Maternal-Fetal Medicine, The Ohio State University, College of Medicine and Public Health, Columbus, Ohio, USA.

出版信息

Am J Obstet Gynecol. 2004 Mar;190(3):809-16. doi: 10.1016/j.ajog.2003.09.052.

DOI:10.1016/j.ajog.2003.09.052

PMID:15042019

Abstract

OBJECTIVE

The objective of this study was to test the hypothesis that peroxisome proliferator activated receptor-gamma (PPAR-gamma) is expressed in intrauterine tissues before active term human parturition, and that its repression is associated with up-regulation of cyclooxygenase-2 (COX-2).

STUDY DESIGN

Specimens were collected from women with term singleton pregnancies after spontaneous labor or cesarean section before labor, prepared for immunoblot and immunohistochemical analysis, and probed for PPAR-gamma or COX-2.

RESULTS

PPAR-gamma expression was prominent in fetal membranes and placenta before active labor. After labor, PPAR-gamma expression was significantly reduced in fetal membranes, but not in placenta. The ratio of COX-2:PPAR-gamma was significantly elevated in fetal membranes with labor. PPAR-gamma immunostaining was prominent in syncytiotrophoblast, extravillous cytotrophoblasts, and cells of the amnion and chorion. COX-2 immunostaining was abundant in the amnion and rare in the placenta.

CONCLUSION

PPAR-gamma is highly expressed in term intrauterine tissues. In fetal membranes, PPAR-gamma levels are reduced once active labor commences, coincidental with a relative increase in COX-2 expression.

摘要