使用次磷酸盐双自旋序弛豫研究人类红细胞的微粘度。
Microviscosity of human erythrocytes studied using hypophosphite two-spin order relaxation.
作者信息
Price W S, Perng B C, Tsai C L, Hwang L P
机构信息
Department of Chemistry, National Taiwan University, Taipei, ROC.
出版信息
Biophys J. 1992 Mar;61(3):621-30. doi: 10.1016/S0006-3495(92)81867-2.
A new 31P NMR method is used to probe the cytoplasmic viscosity of human erythrocytes. The method is based on observing two-spin order relaxation of the 31P atom of the hypophosphite ion. This method is superior to our previous method, using the longitudinal relaxation time of the ion, because random field effects such as intermolecular dipole-dipole relaxation can be separated from intramolecular relaxation. This allows a more accurate determination of the effective reorientational correlation time from the measured intramolecular relaxation because it is now unaffected by random field effects. The new method also provides a means by which to estimate the random field effects. Both two-spin order and proton-decoupled T1 measurements were conducted on hypophosphite in water solutions at various temperatures, glycerol solutions of various viscosities, and in erythrocyte samples of various cell volumes. The results show that the effective reorientational correlation time of the hypophosphite ion varies from 7.2 to 15.2 ps in the cytoplasm of cells ranging in volume from 102 to 56 fl cells.
一种新的31P核磁共振方法被用于探测人类红细胞的细胞质黏度。该方法基于观察次磷酸根离子中31P原子的双自旋序弛豫。此方法优于我们之前使用该离子纵向弛豫时间的方法，因为诸如分子间偶极-偶极弛豫等随机场效应可以与分子内弛豫分离。这使得能够根据测量到的分子内弛豫更准确地确定有效重取向相关时间，因为它现在不受随机场效应的影响。新方法还提供了一种估计随机场效应的手段。在不同温度的水溶液、不同黏度的甘油溶液以及不同细胞体积的红细胞样本中，对次磷酸根进行了双自旋序和质子去耦T1测量。结果表明，在体积从102飞升至56飞升的细胞的细胞质中，次磷酸根离子的有效重取向相关时间在7.2皮秒至15.2皮秒之间变化。
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