Ricote Mónica, García-Tuñón Ignacio, Bethencourt Fermín R, Fraile Benito, Paniagua Ricardo, Royuela Mar
Department of Cell Biology and Genetics, University of Alcalá, Alcalá de Henares, Madrid, Spain.
Cancer. 2004 Apr 1;100(7):1388-96. doi: 10.1002/cncr.20142.
The principal components of the interleukin-1 (IL-1) family are two secreted factors (IL-1alpha and IL-1beta), two transmembrane receptors (IL-1RI [biologically active] and IL-1RII [inert receptor]), and a natural antagonist receptor of IL-1 function (IL-1Ra). Changes in the expression pattern of these IL-1 members have been reported to be related to disease progression. The objective of the current study was to evaluate these changes in prostatic tissue by means of immunohistochemistry and Western blot analysis.
Immunohistochemical and Western blot analyses were performed in 20 normal samples, 35 samples of benign prostatic hyperplasia (BPH) and 27 samples from patients with prostate carcinoma (PC).
In normal prostate samples, immunoreactions to IL-1beta and IL-1RI were positive, whereas there were no immunoreactions observed to IL-1alpha, IL-1RII, or IL-1Ra. In BPH, in addition to immunoreactions to IL-1beta and IL-1RI, immunoreactions to IL-1alpha, IL-1RII, and IL-1Ra were observed in many samples. In samples of PC with low Gleason grade, most tumors had positive immunoreactions to IL-1alpha and IL-1RI. In samples of PC with high Gleason grade, immunoreactions were seen only to IL-1alpha, IL-1RI, and IL-1RII.
The current results suggested that high expression levels of IL-1alpha and IL1-RI in epithelial cells in BPH and PC samples were involved in cell proliferation and that the loss of immunoexpression of IL-1beta and IL-1Ra was a characteristic feature of PC compared with normal prostate samples and BPH. Because this loss is progressive up to a complete absence of immunoexpression in PC of high Gleason grade, the evaluation of IL-1beta and IL-1Ra in PC may be significant in assessing for malignancy.
白细胞介素 -1（IL-1）家族的主要成分包括两种分泌因子（IL-1α和IL-1β）、两种跨膜受体（IL-1RI [具有生物活性]和IL-1RII [无活性受体]）以及一种IL-1功能的天然拮抗剂受体（IL-1Ra）。据报道，这些IL-1家族成员的表达模式变化与疾病进展有关。本研究的目的是通过免疫组织化学和蛋白质印迹分析评估前列腺组织中的这些变化。
对20份正常样本、35份良性前列腺增生（BPH）样本和27份前列腺癌（PC）患者样本进行了免疫组织化学和蛋白质印迹分析。
在正常前列腺样本中，对IL-1β和IL-1RI的免疫反应呈阳性，而对IL-1α、IL-1RII或IL-1Ra未观察到免疫反应。在BPH中，除了对IL-1β和IL-1RI的免疫反应外，在许多样本中还观察到对IL-1α、IL-1RII和IL-1Ra的免疫反应。在低Gleason分级的PC样本中，大多数肿瘤对IL-1α和IL-1RI的免疫反应呈阳性。在高Gleason分级的PC样本中，仅观察到对IL-1α、IL-1RI和IL-1RII的免疫反应。
目前的结果表明，BPH和PC样本上皮细胞中IL-1α和IL1-RI的高表达水平与细胞增殖有关，与正常前列腺样本和BPH相比，IL-1β和IL-1Ra免疫表达的缺失是PC的一个特征。由于这种缺失在高Gleason分级的PC中呈进行性直至完全没有免疫表达，因此评估PC中的IL-1β和IL-1Ra可能对评估恶性肿瘤具有重要意义。
