Koopmanschap B, Lammers H, de Kort S
Department of Entomology, Agricultural University, Wageningen, Netherlands.
Arch Insect Biochem Physiol. 1992;20(2):119-33. doi: 10.1002/arch.940200204.
The protein composition of larval and adult hemolymph from the Colorado potato beetle, Leptinotarsa decemlineata, was investigated and some abundant, high molecular weight proteins were identified and characterized. Diapause protein 1, which occurs in the hemolymph of last instar larvae and short-day adults, appeared to be a storage protein. This protein dissociated into two bands due to the high pH used in nondenaturing gels. Its quaternary structure was established by chemical crosslinking. It appeared to be a hexamer. Diapause protein 1 is composed of approximately 82,000 subunits. The amino acid composition and N-terminal sequence of this protein has been determined. Specific antibodies against diapause protein 1 have been developed. Topical application of 1 microgram pyriproxyfen, a juvenile hormone analog, to last instar larvae and short-day adults suppressed the appearance of this protein in the hemolymph. Pyriproxyfen prematurely induced vitellogenin, when applied to last instar larvae. A larval specific protein was also identified in the hemolymph. Its temporary appearance in the hemolymph of last instar larvae, its subunit composition (M(r) approximately 82,000) and its suppression by pyriproxyfen suggests that this protein is a storage protein as well.
对科罗拉多马铃薯甲虫（Leptinotarsa decemlineata）幼虫和成虫血淋巴的蛋白质组成进行了研究，鉴定并表征了一些丰富的高分子量蛋白质。滞育蛋白1存在于末龄幼虫和短日照成虫的血淋巴中，似乎是一种储存蛋白。由于非变性凝胶中使用的高pH值，该蛋白解离成两条带。通过化学交联确定了其四级结构。它似乎是一种六聚体。滞育蛋白1由大约82,000个亚基组成。已确定该蛋白的氨基酸组成和N端序列。已开发出针对滞育蛋白1的特异性抗体。将1微克吡丙醚（一种保幼激素类似物）局部应用于末龄幼虫和短日照成虫，可抑制该蛋白在血淋巴中的出现。当将吡丙醚应用于末龄幼虫时，它会过早诱导卵黄原蛋白的产生。在血淋巴中还鉴定出一种幼虫特异性蛋白。它在末龄幼虫血淋巴中的短暂出现、其亚基组成（分子量约为82,000）以及被吡丙醚抑制，表明该蛋白也是一种储存蛋白。
