Samaraweera P, Porath J, Law J H
Center for Insect Science, University of Arizona, Tucson 85721.
Arch Insect Biochem Physiol. 1992;20(4):243-51. doi: 10.1002/arch.940200402.
The hemolymph of the adult female Manduca sexta was fractionated by cascade-mode multi-affinity chromatography (CASMAC) on a main-line tandem column chain containing Zn(2+)-TED, T-gel, Ni(2+)-DPA, and phenylsepharose and a side-line column containing Zn(2+)-DPA. The technique separated some of the previously described major hemolymph proteins, and yielded a number of fractions with simple composition. Some of these fractions contained only less abundant proteins of Manduca hemolymph. Thus, it appears that CASMAC would be a very useful fractionation technique for purification and characterization of the minor proteins of insect hemolymph.
成年雌性烟草天蛾的血淋巴通过级联模式多亲和色谱法（CASMAC）在包含Zn(2+)-TED、T-凝胶、Ni(2+)-DPA和苯基琼脂糖的主线串联柱链以及包含Zn(2+)-DPA的侧线柱上进行分离。该技术分离出了一些先前描述的主要血淋巴蛋白，并产生了一些组成简单的组分。其中一些组分仅含有烟草天蛾血淋巴中丰度较低的蛋白质。因此，CASMAC似乎是一种非常有用的分级分离技术，可用于昆虫血淋巴中次要蛋白质的纯化和表征。
