Spengler B, Kirsch D, Kaufmann R, Jaeger E
Institute of Laser Medicine, University of Düsseldorf, Germany.
Rapid Commun Mass Spectrom. 1992 Feb;6(2):105-8. doi: 10.1002/rcm.1290060207.
A novel method of peptide sequencing by mass spectrometry is described. Metastable decay of laser-desorbed ions, taking place in the first field-free drift region of a reflection time-of-flight mass spectrometer, has been monitored to get structural information from larger peptides. Fragment ions from metastable decay are mass analysed by adjusting the potentials of the ion reflectron according to the kinetic energies of the ions. The features of the technique and its significance for future applications are outlined.
描述了一种通过质谱进行肽测序的新方法。监测了在反射式飞行时间质谱仪的第一个无场漂移区域中发生的激光解吸离子的亚稳衰变，以从较大的肽中获取结构信息。通过根据离子的动能调整离子反射镜的电位，对亚稳衰变产生的碎片离子进行质量分析。概述了该技术的特点及其对未来应用的意义。
