Bringmann Gerhard, Lang Gerhard, Steffens Stefan, Schaumann Karsten
Institut für Organische Chemie der Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany.
J Nat Prod. 2004 Mar;67(3):311-5. doi: 10.1021/np034015x.
A strain of Penicillium brevicompactum derived from a specimen of the Mediterranean sponge Petrosia ficiformis was investigated for its secondary metabolites. Using fast centrifugal partitioning chromatography (FCPC) two previously unknown cyclodepsipeptides, petrosifungins A (1) and B (2), were isolated, both containing two neighboring units of the nonproteinogenic amino acid l-pipecolinic acid. The absolute configurations of all amino acids were established to be l using GITC derivatization and Marfey's method. Furthermore, the known metabolite brevianamide A (3) was isolated. The likewise known compounds mycophenolic acid (4) and asperphenamate (5) were identified from their spectroscopic properties directly in the extract using HPLC-UV, -MS, and -NMR coupling.
对从地中海海绵状石海绵（Petrosia ficiformis）标本中分离得到的短密青霉（Penicillium brevicompactum）菌株的次生代谢产物进行了研究。利用快速离心分配色谱法（FCPC）分离出两种此前未知的环缩肽，即石海绵菌素A（1）和B（2），它们均含有两个相邻的非蛋白质氨基酸L-哌啶酸单元。使用GITC衍生化和马尔菲方法确定所有氨基酸的绝对构型均为L型。此外，还分离出了已知的代谢产物短柄酰胺A（3）。同样已知的化合物霉酚酸（4）和曲霉芬酸（5）通过使用HPLC-UV、-MS和-NMR联用技术，根据其光谱性质直接在提取物中鉴定出来。
