Podskochy Alexander, Koulikovska Marina, Fagerholm Per, van der Ploeg Ingeborg
St. Erik's Eye Hospital, Karolinska Institute, Stockholm, Sweden.
Acta Ophthalmol Scand. 2004 Apr;82(2):200-4. doi: 10.1111/j.1600-0420.2004.00232.x.
It is known that stromal proteoglycans play an important role in the hydration and transparency of the mammalian cornea. Proteoglycans have been described as a pathological deposit in climatic proteoglycan stromal keratopathy, which is associated with chronic ultraviolet radiation (UVR) exposure. The expression of dermatan sulfate proteoglycan biglycan in the cornea was thus studied after exposure of rabbit eyes to UVR.
New Zealand albino rabbit corneas were exposed to UVR at 310 nm at the dose producing biomicroscopically significant keratitis (0.47 J/cm2). Animals were killed 3, 7 and 28 days after exposure (five rabbits in each group). Five rabbits were used as controls and did not receive any UVR treatment. Expression of biglycan mRNA in the corneas was investigated by competitive reverse transcription-polymerase chain reaction (RT-PCR).
There was no expression of biglycan mRNA in the control group. In the UVR-exposed groups, biglycan mRNA had still not been expressed 3 days after exposure. The expression of biglycan mRNA was observed in all UVR-treated corneas 7 days after exposure (p < 0.05). By 28 days after UVR exposure the expression of biglycan mRNA had decreased (not statistically significant).
There is no detectable biglycan gene expression in the normal rabbit cornea. Ultraviolet radiation exposure leads to a distinct expression of biglycan mRNA in the rabbit cornea that decreases 4 weeks after exposure, indicating the involvement of biglycan in the corneal repair process. Biglycan appears to be a novel marker of corneal wound healing.
已知基质蛋白聚糖在哺乳动物角膜的水合作用和透明度中起重要作用。蛋白聚糖已被描述为气候性蛋白聚糖性基质角膜病中的一种病理性沉积物，该病与长期紫外线辐射（UVR）暴露有关。因此，在兔眼暴露于UVR后，研究了角膜中硫酸皮肤素蛋白聚糖双糖链蛋白聚糖的表达。
将新西兰白化兔角膜暴露于波长为310 nm的UVR下，剂量为产生具有生物显微镜下显著角膜炎的剂量（0.47 J/cm2）。在暴露后3、7和28天处死动物（每组5只兔子）。5只兔子作为对照，未接受任何UVR处理。通过竞争性逆转录-聚合酶链反应（RT-PCR）研究角膜中双糖链蛋白聚糖mRNA的表达。
对照组中未检测到双糖链蛋白聚糖mRNA的表达。在UVR暴露组中，暴露后3天仍未表达双糖链蛋白聚糖mRNA。暴露后7天，在所有UVR处理的角膜中均观察到双糖链蛋白聚糖mRNA的表达（p<0.05）。到UVR暴露后28天，双糖链蛋白聚糖mRNA的表达有所下降（无统计学意义）。
在正常兔角膜中未检测到双糖链蛋白聚糖基因表达。紫外线辐射暴露导致兔角膜中双糖链蛋白聚糖mRNA明显表达，暴露4周后表达下降，表明双糖链蛋白聚糖参与角膜修复过程。双糖链蛋白聚糖似乎是角膜伤口愈合的一个新标志物。
