外周血循环未成熟细胞计数可预测健康供者在粒细胞集落刺激因子诱导的外周血造血干细胞动员中CD34+细胞的产量。
Peripheral blood circulating immature cell counts predict CD34+ cell yields in G-CSF-induced PBPC mobilization in healthy donors.
作者信息
Kozuka Teruhiko, Ikeda Kazuma, Teshima Takanori, Yoshida Chikamasa, Shinagawa Katsuji, Kojima Kensuke, Matsuo Keitaro, Bessho Akihiro, Sunami Kazutaka, Hiramatsu Yasushi, Maeda Yoshinobu, Noguchi Toshio, Yamamoto Kazuhiko, Fujii Nobuharu, Imai Toshi, Kusumoto Kinuyo Kaneda, Masuda Kozo, Takenaka Katsuto, Ishimaru Fumihiko, Niiya Kenji, Koide Norio, Tanimoto Mitsune, Harada Mine
机构信息
Second Department of Internal Medicine, Division of Blood Transfusion, Okayama University Medical School, Japan.
出版信息
Transfusion. 2004 Apr;44(4):526-32. doi: 10.1111/j.1537-2995.2004.03078.x.
BACKGROUND
It has been previously reported that the number of circulating immature cells (CIC) in peripheral blood (PB) estimates the number of CD34+ cells collected in G-CSF plus chemotherapy-induced PBPC mobilization. The correlation of CIC counts in PB with CD34+ cell yield and its usefulness was evaluated in G-CSF-induced PBPC mobilization for healthy donors.
STUDY DESIGN AND METHODS
CIC counts in PB and CD34+ cell counts in the apheresis product from 122 collections were assessed, and the relationship between these two variables was evaluated with the Pearson rank correlation analysis, the chi-squared test, and the U-test.
RESULTS
CIC counts were correlated weakly with the number of CD34+ cells per L of blood processed in the apheresis product (Pearson rank correlation analysis; r=0.357, p<0.0001). When a level of 1.7 x 10(9) CICs per L was selected as a cutoff value, the sensitivity and specificity for collecting more than 20 x 10(6) CD34+ cells per L of blood processed were 63.6 and 77.5 percent, respectively.
CONCLUSION
The present study suggests that the number of CICs in PB may estimate the number of CD34+ cells collected. The data indicate that CIC counts above 1.7 x 10(9) per L can be used as a good predictor for PBPC collections containing more than 20 x 10(6) CD34+ cells per L of blood processed in a single apheresis procedure.
背景
先前已有报道称，外周血中循环未成熟细胞（CIC）的数量可估算在粒细胞集落刺激因子（G-CSF）加化疗诱导的外周血造血干细胞（PBPC）动员过程中采集的CD34+细胞数量。本研究评估了健康供者在G-CSF诱导的PBPC动员过程中，外周血中CIC计数与CD34+细胞产量的相关性及其实用性。
研究设计与方法
评估了122次采集的外周血中CIC计数和单采产物中的CD34+细胞计数，并采用Pearson等级相关分析、卡方检验和U检验评估这两个变量之间的关系。
结果
CIC计数与单采产物中每升处理血液中的CD34+细胞数量呈弱相关性（Pearson等级相关分析；r = 0.357，p < 0.0001）。当选择每升1.7×10⁹个CIC的水平作为临界值时，每升处理血液中采集超过20×10⁶个CD34+细胞的敏感性和特异性分别为63.6%和77.5%。
结论
本研究表明，外周血中CIC的数量可能估算采集的CD34+细胞数量。数据表明，每升高于1.7×10⁹个的CIC计数可作为在单次单采程序中每升处理血液中含有超过20×10⁶个CD34+细胞的PBPC采集的良好预测指标。
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