Xi Gang-ming, Wang Hua-qiao, He Guo-hou, Huang Chao-fen, Wei Guo-yao
Department of Neurology, Taihe Hospital, Yunyang Medical College, Shiyan 442000, China.
Chin Med J (Engl). 2004 Mar;117(3):389-94.
To date murine models of permanent focal cerebral ischemia have not been well characterized. The purposes of this paper were to compare three different permanent middle cerebral artery occlusion (MCAo) models with or without craniectomy, and to identify an ideal mouse model of permanent focal cerebral ischemia.
Experiments were performed on 45 healthy adult male Kunming mice, weighing 28 to 42 g. The animals were randomly assigned to three groups (n = 15 in every group) based on surgical procedure: MCAo via the external carotid artery (ECA), MCAo via the common carotid artery (CCA), and direct ligation of the middle cerebral artery (MCA). Each day post-ischemia, the animals were scored using an eight-grade neurological function scale, and mortality was also recorded. Seven days post-ischemia, the brains were removed for lesion size determination using triphenyltetrazolium chloride staining. Correlation analysis of lesion volume and neurological score was carried out.
Mortality in the group receiving direct MCA ligation was lowest among the three groups, and there was a significant difference between the direct MCA ligation group and the two intraluminal occlusion groups (P < 0.05). In all groups, neurological scores gradually increased with prolongation of ischemic duration, peaking after two days, then gradually decreasing. In the direct MCA ligation group, however, neurological scores were relatively stable. There was a significant correlation between infarct volume and neurological score 7 days after MCAo in every group (all r > 0.7, P < 0.05), suggesting good reproducibility of lesion volume in the three groups, but the infarct volume was more constant in the direct MCA ligation group.
The direct ligation model of MCAo provides an optimal means of studying permanent focal cerebral ischemia, and is preferable to the models using intraluminal sutures.
迄今为止，永久性局灶性脑缺血的小鼠模型尚未得到充分表征。本文的目的是比较三种不同的永久性大脑中动脉闭塞（MCAo）模型（有无颅骨切除术），并确定一种理想的永久性局灶性脑缺血小鼠模型。
对45只体重28至42克的健康成年雄性昆明小鼠进行实验。根据手术方法将动物随机分为三组（每组n = 15）：经颈外动脉（ECA）进行MCAo、经颈总动脉（CCA）进行MCAo以及直接结扎大脑中动脉（MCA）。缺血后每天，使用八级神经功能量表对动物进行评分，并记录死亡率。缺血7天后，取出大脑，使用氯化三苯基四氮唑染色确定病变大小。对病变体积和神经评分进行相关性分析。
直接MCA结扎组的死亡率在三组中最低，直接MCA结扎组与两个腔内闭塞组之间存在显著差异（P < 0.05）。在所有组中，神经评分随缺血时间延长逐渐增加，在两天后达到峰值，然后逐渐下降。然而，在直接MCA结扎组中，神经评分相对稳定。每组MCAo 7天后梗死体积与神经评分之间存在显著相关性（所有r > 0.7，P < 0.05），表明三组病变体积的重复性良好，但直接MCA结扎组的梗死体积更恒定。
MCAo的直接结扎模型为研究永久性局灶性脑缺血提供了一种最佳方法，优于使用腔内缝线的模型。
