Yang Li-ye, Liu Xiang-ming, Sun Bing, Hui Guo-zhen, Fei Jian, Guo Li-he
Department of Neurosurgery, First Affiliated Hospital, Suzhou University, Suzhou 215006, China.
Chin Med J (Engl). 2004 Mar;117(3):425-9.
Adipose tissue-derived stromal cells (ADSCs) can be greatly expanded in vitro, and induced to differentiate into multiple mesenchymal cell types, including osteogenic, chondrogenic, myogenic, and adipogenic cells. This study was designed to investigate the possibility of ADSCs differentiating into neurons.
Adipose tissue from rats was digested with collagenase, and adherent stromal cells were cultured. A medium containing a low concentration of fetal bovine serum was adopted to induce the cells to differentiate. ADSCs were identified by immunocytochemistry, and semi-quantitative RT-PCR was applied to detect mRNA expression of neurofilament 1 (NF1), nestin, and neuron-specific enolase (NSE).
Nestin-positive cells were found occasionally among ADSCs. ADSCs were found to express NSE mRNA and nestin mRNA, but not NF1 mRNA. ADSCs could differentiate into neuron-like cells in a medium composed of a low concentration of fetal bovine serum, and these differentiated cells displayed complicated neuron-like morphologies.
The data support the hypothesis that adipose tissue contains stem cells capable of differentiating into neurons. These stem cells can overcome their mesenchymal commitment, and may represent an alternative autologous stem cell source for CNS cell transplantation.
脂肪组织来源的基质细胞（ADSCs）可在体外大量扩增，并诱导分化为多种间充质细胞类型，包括成骨细胞、软骨细胞、肌细胞和脂肪细胞。本研究旨在探讨ADSCs分化为神经元的可能性。
用胶原酶消化大鼠脂肪组织，培养贴壁基质细胞。采用含低浓度胎牛血清的培养基诱导细胞分化。通过免疫细胞化学鉴定ADSCs，并应用半定量RT-PCR检测神经丝1（NF1）、巢蛋白和神经元特异性烯醇化酶（NSE）的mRNA表达。
在ADSCs中偶尔发现巢蛋白阳性细胞。ADSCs表达NSE mRNA和巢蛋白mRNA，但不表达NF1 mRNA。ADSCs在含低浓度胎牛血清培养基中可分化为神经元样细胞，这些分化细胞呈现复杂的神经元样形态。
数据支持脂肪组织含有能够分化为神经元的干细胞这一假说。这些干细胞可克服其间充质定向分化，可能成为中枢神经系统细胞移植的另一种自体干细胞来源。
