Hamdan H F, Luikart S D
Department of Medicine, University of Minnesota Medical School, Minneapolis.
Oncol Res. 1992;4(4-5):201-7.
The extracellular matrix influences the growth and differentiation of a variety of cell types. In this study, the effects of bone marrow extracellular matrix on U-937 cells, a human histiocytic lymphoma cell line, were assessed. Sixty percent of U-937 cells adhered to extracellular matrix, whereas only 1% adhered to uncoated plastic. U-937 cells grown on extracellular matrix released significantly more lysozyme into the medium (8.3 +/- 0.3 micrograms/10(6) cells) compared to those grown on plastic (4.2 +/- 0.5 micrograms/10(6) cells). FMLP (f-met-leu-phe) receptor expression was also enhanced suggesting a more mature phenotype in cells grown on matrix (2980 cpm/10(6) cells vs 230 cpm/10(6) cells on plastic). Furthermore, bone marrow extracellular matrix inhibited proliferation of U-937 cells. After four days in culture, there was a 65% inhibition of cell growth in matrix-coated flasks compared to uncoated flasks. Since an arrest in G0/G1 usually precedes mammalian cell differentiation, DNA histograms were performed on U-937 cells grown on matrix to detect such an arrest. However, the cell cycle distribution of U-937 cells grown on extracellular matrix or uncoated plastic for various time periods was similar. In contrast, bromodeoxyuridine pulse labeling revealed approximately a 5 hr prolongation in cycle length in cells grown on extracellular matrix. We conclude that bone marrow extracellular matrix induced macrophage-like differentiation and inhibited proliferation of U-937 cells with a prolongation of the cell cycle that was not G0/G1 phase specific.
细胞外基质影响多种细胞类型的生长和分化。在本研究中，评估了骨髓细胞外基质对人组织细胞淋巴瘤细胞系U-937细胞的影响。60%的U-937细胞黏附于细胞外基质，而仅有1%黏附于未包被的塑料表面。与在塑料表面生长的U-937细胞相比，在细胞外基质上生长的U-937细胞向培养基中释放的溶菌酶显著更多（8.3±0.3微克/10⁶个细胞 vs 4.2±0.5微克/10⁶个细胞）。甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）受体表达也增强，提示在基质上生长的细胞具有更成熟的表型（2980 cpm/10⁶个细胞 vs 塑料表面的230 cpm/10⁶个细胞）。此外，骨髓细胞外基质抑制U-937细胞的增殖。培养四天后，与未包被的培养瓶相比，基质包被的培养瓶中细胞生长受到65%的抑制。由于哺乳动物细胞分化通常先出现G0/G1期停滞，因此对在基质上生长的U-937细胞进行了DNA直方图分析以检测这种停滞。然而，在细胞外基质或未包被塑料上生长不同时间段的U-937细胞的细胞周期分布相似。相反，溴脱氧尿苷脉冲标记显示，在细胞外基质上生长的细胞周期长度延长约5小时。我们得出结论，骨髓细胞外基质诱导U-937细胞向巨噬细胞样分化并抑制其增殖，细胞周期延长但并非G0/G1期特异性。
