Tseng Wen-Fang, Huang Shuan Shian, Huang Jung San
Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Boulevard, St. Louis, MO 63104, USA.
FEBS Lett. 2004 Mar 26;562(1-3):71-8. doi: 10.1016/S0014-5793(04)00185-1.
The type V transforming growth factor-beta (TGF-beta) receptor (TbetaR-V) is hypothesized to be involved in cellular growth inhibition by TGF-beta(1). Recently, TbetaR-V was found to be identical to low density lipoprotein receptor-related protein-1 (LRP-1). Here we demonstrate that TGF-beta(1) inhibits growth of wild-type CHO cells but not LRP-1-deficient mutant cells (CHO-LRP-1(-) cells). Stable transfection of CHO-LRP-1(-) cells with LRP-1 cDNA restores the wild-type morphology and the sensitivity to growth inhibition by TGF-beta(1). In addition, overexpression of LRP-1 minireceptors exerts a dominant negative effect and attenuates the growth inhibitory response to TGF-beta(1) in wild-type CHO cells. These results suggest that LRP-1/TbetaR-V is critical for TGF-beta(1)-mediated growth inhibition in CHO cells.
V型转化生长因子-β（TGF-β）受体（TβR-V）被推测参与TGF-β(1)介导的细胞生长抑制。最近，发现TβR-V与低密度脂蛋白受体相关蛋白-1（LRP-1）相同。在此我们证明，TGF-β(1)抑制野生型CHO细胞的生长，但不抑制LRP-1缺陷型突变细胞（CHO-LRP-1(-)细胞）的生长。用LRP-1 cDNA稳定转染CHO-LRP-1(-)细胞可恢复野生型形态以及对TGF-β(1)介导的生长抑制的敏感性。此外，LRP-1微型受体的过表达发挥显性负效应，并减弱野生型CHO细胞对TGF-β(1)的生长抑制反应。这些结果表明，LRP-1/TβR-V对CHO细胞中TGF-β(1)介导的生长抑制至关重要。
