Sood P P, Lodha M
J Hirnforsch. 1978;19(1):5-20.
In the present contribution a comparative study of histochemical mapping of the distribution of adenosine triphosphatase and 5-nucleotidase in the hind brain of mouse has been made. There are many similarities and dissimilarities between the distribution of these two enzymes in various nuclei, tracts and fiber bundles. The noteworthy differences are as follows:--1. The AP, NDNV, NI, NNH, NOAD, NOAM, NOI NPC, TS and SG are very intensely positive for ATPase whereas, in 5-NUC study none of these nuclei demonstrates intensity of such degree. 2. Nucleus ambiguus is intensely positive for ATPase and is completely negative for 5-NUC. 3. The nucleus n. facialis is intensely positive for ATPase and is moderately positive for 5-NUC. 4. NC, GN, PN, PCI and TC are completely negative for 5-NUC. In ATPase preparations only GN is negative and the rest of the areas demonstrate intensity of various degrees. Along with these differences, similarities in the intensity of both enzymes in various nuclei, tracts and fibrous bundles also exist. An attempt has been made to correlate all the aforesaid differences and similarities in the distribution of these two enzymes with the functional nature of the various areas of hind brain of mouse.
本研究对小鼠后脑三磷酸腺苷酶和5-核苷酸酶分布的组织化学图谱进行了比较研究。这两种酶在不同核团、神经束和纤维束中的分布存在许多异同。值得注意的差异如下:1. 后极核、舌下神经核腹内侧部、疑核、孤束核、下橄榄核背侧副核、下橄榄核内侧副核、下橄榄核中间核、脑桥核、顶盖脊髓束和脊髓灰质连合在三磷酸腺苷酶染色中呈强阳性,而在5-核苷酸酶研究中,这些核团均未显示出如此程度的阳性强度。2. 疑核在三磷酸腺苷酶染色中呈强阳性,在5-核苷酸酶染色中完全阴性。3. 面神经核在三磷酸腺苷酶染色中呈强阳性,在5-核苷酸酶染色中呈中度阳性。4. 颈核、巨细胞网状核、脑桥网状核、小细胞网状核和延髓尾侧网状核在5-核苷酸酶染色中完全阴性。在三磷酸腺苷酶染色制剂中,只有巨细胞网状核呈阴性,其余区域显示出不同程度的阳性强度。除了这些差异外,这两种酶在不同核团、神经束和纤维束中的阳性强度也存在相似之处。已尝试将上述两种酶分布的所有差异和相似之处与小鼠后脑各区域的功能性质联系起来。