Dabiri Shahriar, Huntsman David, Makretsov Nikita, Cheang Maggie, Gilks Blake, Bajdik Chris, Gelmon Karen, Chia Stephen, Hayes Malcom
Department of Pathology, British Columbia Cancer Agency, Vancouver, BC, Canada.
Mod Pathol. 2004 Jun;17(6):690-5. doi: 10.1038/modpathol.3800094.
Tissue microarrays containing 348 cases of invasive breast carcinoma were studied by immunohistochemical staining for CD-117, CD-3, CD-20, CD-68, Her2, estrogen receptor protein, and progesterone receptor protein, and results were correlated with patient outcome. Hormone receptor status (both estrogen receptor and progesterone receptor) correlated with a good outcome while Her2 overexpression was associated with a poor outcome. The presence of mast cells in the stroma, as demonstrated by positive c-kit (CD-117) staining, correlated with a good prognosis (P=0.0036). On subset analysis, this association between the presence of mast cells and favorable prognosis was present in the node-negative patients (P=0.018). The presence of mast cells showed an inverse correlation with the presence of CD-68 positive macrophages. No correlation was observed between the presence of mast cells and either B-cells (CD20-positive) or T-cells (CD3-positive). The presence of stromal mast cells was of prognostic significance independent of nodal status and tumor size (P=0.02). When the multivariate analysis was expanded to include tumor grade, estrogen receptor status and Her2 status, as well as tumor size and nodal status, the presence of stromal mast cells approached significance as an independent prognostic indicator.
通过对含有348例浸润性乳腺癌的组织芯片进行CD - 117、CD - 3、CD - 20、CD - 68、Her2、雌激素受体蛋白和孕激素受体蛋白的免疫组织化学染色研究，并将结果与患者预后相关联。激素受体状态（雌激素受体和孕激素受体均为阳性）与良好预后相关，而Her2过表达与不良预后相关。基质中肥大细胞的存在，通过c - kit（CD - 117）阳性染色证实，与良好预后相关（P = 0.0036）。在亚组分析中，肥大细胞的存在与良好预后之间的这种关联在无淋巴结转移的患者中存在（P = 0.018）。肥大细胞的存在与CD - 68阳性巨噬细胞的存在呈负相关。未观察到肥大细胞的存在与B细胞（CD20阳性）或T细胞（CD3阳性）之间存在相关性。基质肥大细胞的存在具有独立于淋巴结状态和肿瘤大小的预后意义（P = 0.02）。当多因素分析扩展到包括肿瘤分级、雌激素受体状态、Her2状态以及肿瘤大小和淋巴结状态时，基质肥大细胞作为独立预后指标接近显著性。
