Wambura C, Aoyama N, Shirasaka D, Kuroda K, Watanabe Y, Miki I, Tamura T, Kasuga M
Division of Diabetes, Digestive and Kidney Diseases, Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, Kobe, Hyogo 650-0017, Japan.
Dig Liver Dis. 2004 Mar;36(3):178-86. doi: 10.1016/j.dld.2003.10.014.
Proliferation and apoptosis events are altered in Helicobacter pylori infection. However, whether H. pylori eradication has an effect on the disturbed kinetics in metaplastic mucosa has not been well elucidated.
To investigate the effect of eradication on the gastric cell kinetics.
Initially, biopsies were obtained from 74 H. pylori-infected subjects and repeated 12 and 24 months after eradication. Biopsies were immunohistochemically stained for apoptosis by single-stranded DNA, for proliferation by Ki-67 antibodies and for intestinal metaplasia MUC2, MUC5AC, MUC6 and CD10.
While antral apoptosis in intestinal metaplasia was significantly lower than in non-intestinal metaplasia, proliferation was significantly higher (greater and lesser curvatures, P < 0.05, respectively). This resulted in a significantly lower apoptosis/proliferation ratio in intestinal metaplasia than in non-intestinal metaplasia (antrum greater and lesser curvatures and corpus greater curvature, P < 0.05). After successful eradication, apoptosis and proliferation decreased in both intestinal metaplasia and non-intestinal metaplasia. The pattern of reduction of apoptosis and proliferation differed in these two groups. However, in the corpus, the reduction resulted in a significant increase in the apoptosis/proliferation ratio in both.
Proliferation and apoptosis are unevenly and disproportionately altered in H. pylori infection leading to an imbalance in cell kinetics. Eradication of the organism improves the balance and may possibly play a role in the prevention of malignancy transformation in the metaplastic mucosa.
幽门螺杆菌感染会改变增殖和凋亡事件。然而，幽门螺杆菌根除对化生黏膜中紊乱的动力学是否有影响尚未得到充分阐明。
研究根除幽门螺杆菌对胃细胞动力学的影响。
最初，从74名幽门螺杆菌感染患者获取活检样本，并在根除后12个月和24个月重复取样。活检样本通过单链DNA进行凋亡免疫组织化学染色，通过Ki-67抗体进行增殖染色，通过MUC2、MUC5AC、MUC6和CD10进行肠化生染色。
肠化生中的胃窦凋亡显著低于非肠化生，但增殖显著更高（大弯和小弯处，P值分别<0.05）。这导致肠化生中的凋亡/增殖比显著低于非肠化生（胃窦大弯和小弯以及胃体大弯处，P<0.05）。成功根除后，肠化生和非肠化生中的凋亡和增殖均下降。这两组中凋亡和增殖的下降模式不同。然而，在胃体中，这种下降导致两者的凋亡/增殖比均显著增加。
幽门螺杆菌感染中增殖和凋亡发生不均衡且不成比例的改变，导致细胞动力学失衡。根除该病原体可改善这种平衡，并可能在预防化生黏膜的恶性转化中发挥作用。
