Yamasaki Alzira, Cunha M Angela S D A, Oliveira João A B P, Duarte Armando C, Gomes M Teresa S R
Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Biosens Bioelectron. 2004 May 15;19(10):1203-8. doi: 10.1016/j.bios.2003.11.005.
A piezoelectric quartz crystal microbalance has been shown to be useful to monitor real time bacterial growth. Monitoring bacterial growth can give an insight into the ecosystem, as it is highly affected by the presence of toxic elements or nutrients. The frequency of an uncoated piezoelectric quartz crystal was monitored while in contact with bacteria, isolated from water sampled from a Portuguese lagoon, growing in two different media: a saline nutrient broth (NM) and the natural water. The sensor was used to evaluate the effect of copper on bacterial growth. Copper concentrations up to 18.8 microg l(-1) showed an increase in bacterial growth in NM, and a decrease beyond 25.0 microg l(-1). Copper added to the natural water had negative effects on bacterial growth beyond 18.8 microg l(-1). Copper concentrations in the natural water from the lagoon were determined using a similar quartz crystal to detect the mass deposited by anodic stripping voltammetry, and was found to be 3.38 +/- 0.09 microg l(-1).
已证明压电石英晶体微天平可用于实时监测细菌生长。监测细菌生长有助于深入了解生态系统，因为它极易受到有毒元素或营养物质存在的影响。在与从葡萄牙泻湖采集的水样中分离出的细菌接触时，监测未涂层压电石英晶体的频率，这些细菌在两种不同培养基中生长：盐性营养肉汤（NM）和天然水。该传感器用于评估铜对细菌生长的影响。在NM中，铜浓度高达18.8微克/升时细菌生长增加，超过25.0微克/升时则减少。添加到天然水中的铜在超过18.8微克/升时对细菌生长有负面影响。使用类似的石英晶体通过阳极溶出伏安法检测沉积质量来测定泻湖天然水中的铜浓度，结果发现为3.38±0.09微克/升。
