Diessel Edgar, Grothe Klaus, Siebert Hans-Michael, Warner Brian D, Burmeister Jens
Bayer AG, Bayer Technology Services, Competence Center Biophysics, Building E41, 51368 Leverkusen, Germany.
Biosens Bioelectron. 2004 May 15;19(10):1229-35. doi: 10.1016/j.bios.2003.11.013.
DNA diagnostics at the point-of-care requires biosensors that rely on highly sensitive transducers and are producible at low cost. A promising candidate technology is based on direct electrical detection of autometallographically enhanced Au labeled analytes. We present a substantial improvement to the previously used method by introducing online DC resistance monitoring during the autometallographic enhancement process. Since multi-step enhancement, washing, drying, and measurement cycles are eliminated, our method takes the direct electrical detection method a step further to applicability in a point-of-care environment. The feasibility of the novel method is demonstrated by its application in a simple DNA hybridization assay and the analysis of a single nucleotide polymorphism (SNP) using allele-specific hybridization. Unequivocal discrimination of all possible base pairing combinations in the SNP assay has been achieved. The SNP assay in particular indicates the potential of the method for analyte quantification.
即时医疗诊断中的DNA检测需要依赖高灵敏度传感器且能低成本生产的生物传感器。一种有前景的候选技术是基于对自动金相增强金标记分析物的直接电学检测。我们通过在自动金相增强过程中引入在线直流电阻监测，对先前使用的方法进行了重大改进。由于消除了多步增强、洗涤、干燥和测量循环，我们的方法使直接电学检测方法在即时医疗环境中的适用性更进一步。该新方法的可行性通过其在简单DNA杂交检测中的应用以及使用等位基因特异性杂交对单核苷酸多态性（SNP）的分析得到了证明。在SNP检测中已实现对所有可能碱基配对组合的明确区分。特别是SNP检测表明了该方法在分析物定量方面的潜力。
