通过等电聚焦凝胶电泳对炭疽芽孢杆菌外毒素成分进行蛋白质印迹分析。

Western blot analysis of the exotoxin components from Bacillus anthracis separated by isoelectric focusing gel electrophoresis.

作者信息

Little Stephen F

机构信息

Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, MD 21702-5011, USA.

出版信息

Biochem Biophys Res Commun. 2004 Apr 23;317(1):294-300. doi: 10.1016/j.bbrc.2004.03.044.

DOI:10.1016/j.bbrc.2004.03.044

PMID:15047182

Abstract

The components of the Bacillus anthracis exotoxins, protective antigen (PA), lethal factor (LF), and edema factor (EF), from 24 isolates were separated by isoelectric focusing gel electrophoresis and detected by Western blot with monoclonal antibodies. Only two isoforms each were observed for PA and EF. Four isoforms were identified for LF. The biological activities of both lethal toxin and edema toxin were measured by using in vitro cell-based assays. This study provides another method of characterizing various isolates of B. anthracis by determining the isoelectric points of the exotoxin components and may be useful in the development of protective vaccines against B. anthracis infection.

摘要

采用等电聚焦凝胶电泳法对24株炭疽芽孢杆菌分离株的炭疽芽孢杆菌外毒素成分，即保护性抗原（PA）、致死因子（LF）和水肿因子（EF）进行分离，并用单克隆抗体通过蛋白质印迹法进行检测。PA和EF各自仅观察到两种亚型。LF鉴定出四种亚型。通过基于细胞的体外试验测定致死毒素和水肿毒素的生物活性。本研究通过测定外毒素成分的等电点，提供了另一种鉴定炭疽芽孢杆菌不同分离株的方法，可能有助于开发针对炭疽芽孢杆菌感染的保护性疫苗。