Charcosset C, Fiaty K, Perrin B, Couturier R, Maïsterrena B
Laboratoire d'Automatique et de Génie des Procédés, UMR-CNRS 5007, CPE Lyon, France.
Arch Biochem Biophys. 2004 Apr 15;424(2):235-45. doi: 10.1016/j.abb.2004.02.016.
The coupling of diffusion, reaction, and electrostatic interactions existing in the microenvironment of permeable and charged bi-enzymatic membranes has led to the concept of biomimetic membranes. These membranes permit the active and specific transport of small hydrophilic molecules against their concentration gradients, at constant temperature and pressure. This study shows that such membranes behave in totally different ways, depending both on the relative position of the two enzymes, either within or outside the ionic double layer, and on the membrane surface potentials.
可渗透且带电的双酶膜微环境中存在的扩散、反应和静电相互作用的耦合催生了仿生膜的概念。这些膜能够在恒定的温度和压力下，使亲水性小分子逆浓度梯度进行主动且特异性的运输。这项研究表明，此类膜的行为方式截然不同，这既取决于两种酶在离子双层内或外的相对位置，也取决于膜表面电位。
