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在无菌小鼠模型中，使用组特异性16S rRNA探针结合流式细胞术通过荧光杂交评估阿莫西林-克拉维酸对人粪便菌群的影响。

Effect of amoxicillin-clavulanic acid on human fecal flora in a gnotobiotic mouse model assessed with fluorescence hybridization using group-specific 16S rRNA probes in combination with flow cytometry.

作者信息

Barc Marie Claude, Bourlioux François, Rigottier-Gois Lionel, Charrin-Sarnel Céline, Janoir Claire, Boureau Hélène, Doré Joël, Collignon Anne

机构信息

Unité associée INRA Ecosystème Microbien Digestif et Santé, Faculté de Pharmacie, Université Paris-Sud, 92296 Châtenay-Malabry Cedex, France.

出版信息

Antimicrob Agents Chemother. 2004 Apr;48(4):1365-8. doi: 10.1128/AAC.48.4.1365-1368.2004.

DOI:10.1128/AAC.48.4.1365-1368.2004
PMID:15047545
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC375311/
Abstract

Predominant groups of bacteria from a human fecal flora-associated mouse model challenged with amoxicillin-clavulanic acid were quantified with fluorescence in situ hybridization combined with flow cytometry using specific 16S rRNA targeted oligonucleotide probes. This approach provides a useful tool with high throughput to evaluate fecal microflora under antibiotic treatment.

摘要

利用针对16S rRNA的特异性寡核苷酸探针，通过荧光原位杂交结合流式细胞术，对用阿莫西林-克拉维酸攻击的人粪便菌群相关小鼠模型中的主要细菌菌群进行了定量分析。这种方法提供了一种高通量的有用工具，用于评估抗生素治疗下的粪便微生物群。

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本文引用的文献

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Fluorescent hybridisation combined with flow cytometry and hybridisation of total RNA to analyse the composition of microbial communities in human faeces using 16S rRNA probes.荧光杂交结合流式细胞术以及总RNA杂交，使用16S rRNA探针分析人类粪便中微生物群落的组成。
FEMS Microbiol Ecol. 2003 Mar 1;43(2):237-45. doi: 10.1111/j.1574-6941.2003.tb01063.x.
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Growth requirements and fermentation products of Fusobacterium prausnitzii, and a proposal to reclassify it as Faecalibacterium prausnitzii gen. nov., comb. nov.普氏栖粪杆菌的生长需求和发酵产物，以及将其重新分类为普氏粪杆菌属（新属）、新组合的提议
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Ecological effects of linezolid versus amoxicillin/clavulanic acid on the normal intestinal microflora.利奈唑胺与阿莫西林/克拉维酸对正常肠道微生物群的生态效应。
Scand J Infect Dis. 2001;33(12):899-903. doi: 10.1080/00365540110076714.
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A molecular view of the intestinal ecosystem.肠道生态系统的分子视角。
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Comparative study of bacterial groups within the human cecal and fecal microbiota.人体盲肠和粪便微生物群中细菌类群的比较研究。
Appl Environ Microbiol. 2001 Oct;67(10):4939-42. doi: 10.1128/AEM.67.10.4939-4942.2001.
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Fusobacterium prausnitzii and related species represent a dominant group within the human fecal flora.普氏栖粪杆菌及相关菌种是人类粪便菌群中的优势菌群。
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Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization.通过寡核苷酸探针杂交对人类粪便菌群中的细菌类群进行定量分析。
Appl Environ Microbiol. 2000 May;66(5):2263-6. doi: 10.1128/AEM.66.5.2263-2266.2000.
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Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut.对来自复杂群落的编码16S rRNA的基因进行直接分析，揭示了人类肠道内许多新的分子物种。
Appl Environ Microbiol. 1999 Nov;65(11):4799-807. doi: 10.1128/AEM.65.11.4799-4807.1999.
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Functional food science and gastrointestinal physiology and function.功能性食品科学与胃肠生理学及功能
Br J Nutr. 1998 Aug;80 Suppl 1:S147-71. doi: 10.1079/bjn19980108.
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Creating and maintaining the gastrointestinal ecosystem: what we know and need to know from gnotobiology.创建和维持胃肠道生态系统：悉生生物学的已知与未知
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