在无菌小鼠模型中，使用组特异性16S rRNA探针结合流式细胞术通过荧光杂交评估阿莫西林-克拉维酸对人粪便菌群的影响。
Effect of amoxicillin-clavulanic acid on human fecal flora in a gnotobiotic mouse model assessed with fluorescence hybridization using group-specific 16S rRNA probes in combination with flow cytometry.
作者信息
Barc Marie Claude, Bourlioux François, Rigottier-Gois Lionel, Charrin-Sarnel Céline, Janoir Claire, Boureau Hélène, Doré Joël, Collignon Anne
机构信息
Unité associée INRA Ecosystème Microbien Digestif et Santé, Faculté de Pharmacie, Université Paris-Sud, 92296 Châtenay-Malabry Cedex, France.
出版信息
Antimicrob Agents Chemother. 2004 Apr;48(4):1365-8. doi: 10.1128/AAC.48.4.1365-1368.2004.
Abstract
Predominant groups of bacteria from a human fecal flora-associated mouse model challenged with amoxicillin-clavulanic acid were quantified with fluorescence in situ hybridization combined with flow cytometry using specific 16S rRNA targeted oligonucleotide probes. This approach provides a useful tool with high throughput to evaluate fecal microflora under antibiotic treatment.
摘要
利用针对16S rRNA的特异性寡核苷酸探针，通过荧光原位杂交结合流式细胞术，对用阿莫西林-克拉维酸攻击的人粪便菌群相关小鼠模型中的主要细菌菌群进行了定量分析。这种方法提供了一种高通量的有用工具，用于评估抗生素治疗下的粪便微生物群。
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