Skiri H T, Galizia C G, Mustaparta H
Norwegian University of Science and Technology, Department of Biology, Neuroscience Unit, Olav Kyrres gate 3, NO-7489 Trondheim, Norway.
Chem Senses. 2004 Mar;29(3):253-67. doi: 10.1093/chemse/bjh026.
The primary olfactory centre, the antennal lobe of Heliothis virescens moths, contains 62 glomeruli which process plant odour information and four male-specific glomeruli which form the macroglomerular complex, involved in processing information about pheromone and interspecific signals. Using calcium imaging, we recorded the spatio-temporal activity pattern of the glomeruli in the anterior antennal lobe during stimulation with odorants produced by plants or insects. Each odorant elicited specific excitatory responses in one or a few glomeruli: the major pheromone component did so exclusively in the large glomerulus of the macroglomerular complex and the plant odours exclusively in the ordinary glomeruli. Eight glomeruli, with corresponding plant odour responses and positions, were identified within each sex. Glomeruli responded specifically to linalool, beta-ocimene/beta-myrcene or germacrene D/alpha-farnesene. Responses to two essential plant oils covered the response areas of their major constituents, as well as activating additional glomeruli. Stronger activation in the AL due to increased odour concentration was expressed as increased response strength within the odorant-specific glomeruli as well as recruitment of less sensitive glomeruli.
主要嗅觉中枢，即棉铃虫蛾的触角叶，包含62个处理植物气味信息的小球和四个形成大球复合体的雄性特异性小球，大球复合体参与处理有关信息素和种间信号的信息。我们使用钙成像技术，记录了在用植物或昆虫产生的气味剂刺激期间触角叶前部小球的时空活动模式。每种气味剂在一个或几个小球中引发特定的兴奋性反应：主要信息素成分仅在大球复合体的大球中引发反应，而植物气味仅在普通小球中引发反应。在每个性别中鉴定出八个具有相应植物气味反应和位置的小球。小球对芳樟醇、β-罗勒烯/β-月桂烯或大根香叶烯D/α-法呢烯有特异性反应。对两种植物精油的反应覆盖了其主要成分的反应区域，同时还激活了其他小球。由于气味浓度增加而在触角叶中产生的更强激活表现为在气味剂特异性小球内反应强度增加以及对不太敏感的小球的募集。
