使用酶联免疫吸附测定法定量测定脑脊液中的转铁蛋白。

Quantitative determination of transferrin in cerebrospinal fluid using an enzyme linked immunosorbent assay.

作者信息

Van Landeghem G F, D'Haese P C, Lamberts L V, De Broe M E

机构信息

University of Antwerp, Department of Nephrology-Hypertension, University Hospital Antwerp, Wilrijkstraat 10, B-2650, Edegem/Antwerpen, Belgium.

出版信息

Anal Bioanal Chem. 1996 Apr;355(1):96-7. doi: 10.1007/s0021663550096.

DOI:10.1007/s0021663550096

PMID:15048385

Abstract

An enzyme linked immunosorbent assay (ELISA) based method for the determination of transferrin in cerebrospinal fluid (CSF) is described. This method allows transferrin determinations in ultra-small (</= 5 microL) sample volumes. The proposed method offers the possibility to determine transferrin at very low concentrations at which existing methodologies fail because of their insufficient detection limits. The accuracy of the proposed technique was validated by comparing the results of 20 CSF samples obtained by ELISA to those measured by nephelometry yielding mean +/- SD values of 21.1+/-5 mg/L and 20.6 +/- 6 mg/L, respectively (y = 1.13X - 2.97, r = 0.899). The interassay CV was below 10% whereas the detection limit was 2.9 microg/L.

摘要

本文描述了一种基于酶联免疫吸附测定（ELISA）的方法，用于测定脑脊液（CSF）中的转铁蛋白。该方法能够在超小（≤5微升）样本体积中测定转铁蛋白。所提出的方法提供了在极低浓度下测定转铁蛋白的可能性，而现有方法由于检测限不足，在这些极低浓度下无法进行测定。通过将ELISA法检测的20份脑脊液样本结果与散射比浊法测定结果进行比较，验证了所提技术的准确性，ELISA法和散射比浊法的平均±标准差分别为21.1±5毫克/升和20.6±6毫克/升（y = 1.13X - 2.97，r = 0.899）。批间变异系数低于10%，而检测限为2.9微克/升。