Lin J M, Nakagama T, Okazawa H, Wu X Z, Hobo T
Department of Industrial Chemistry, Faculty of Engineering, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, 192-03, Hachioji, Tokyo, Japan.
Anal Bioanal Chem. 1996 Feb;354(4):451-4. doi: 10.1007/s0021663540451.
Capillary gel electrophoresis (CGE) was successfully applied to the separation of optically active isomers and position isomers by incorporating a suitable cyclodextrin chiral selector in polyacrylamide gel. A commercially available ss-cyclodextrin (ss-CD) was used for enantioselectivity towards o-, m- and p-nitrobenzoic acid, o-, m- and p-hydroxybenzoic acid, o-, m- and p-toluic acid and the optical isomers of dansyl-D,L-leucine and R,S-1,1'-binaphthyl-2,2-dihydrogenphosphate. Especially the effect of organic solvents, such as acetonitrile, methanol, dimethylsulphoxide and others were examined in detail. The resolution varied to some extent with the addition of the organic solvent to the polyacrylamide gel and the running buffer solution. The possible mechanism has also been discussed. In addition, quantitative aspects of the separation of stereoisomers using CGE have been studied, showing that both the resolution and accuracy of the determinations were affected by the ratio of the enantiomers.
通过在聚丙烯酰胺凝胶中加入合适的环糊精手性选择剂，毛细管凝胶电泳（CGE）成功应用于旋光异构体和位置异构体的分离。使用市售的β-环糊精（β-CD）对对硝基苯甲酸、间硝基苯甲酸、邻硝基苯甲酸、对羟基苯甲酸、间羟基苯甲酸、邻羟基苯甲酸、对甲苯酸、间甲苯酸、邻甲苯酸以及丹磺酰-D,L-亮氨酸和R,S-1,1'-联萘-2,2-二氢磷酸酯的旋光异构体进行对映体选择性研究。特别详细研究了乙腈、甲醇、二甲基亚砜等有机溶剂的影响。随着向聚丙烯酰胺凝胶和运行缓冲溶液中添加有机溶剂，分离度在一定程度上有所变化。还讨论了可能的机理。此外，对使用CGE分离立体异构体的定量方面进行了研究，结果表明对映体比例会影响测定的分离度和准确性。
