Khlebovich V V, Kulangieva L V, Degtiarev A V
Zoological Institute, Russian Academy of Sciences, Universitetskaya nab. 1, St. Petersburg, 199034 Russia.
Izv Akad Nauk Ser Biol. 2004 Jan-Feb(1):5-7.
Salinity acclimation of euryhaline ciliate Paramecium calkinsi evaluated by swimming velocity was over in 4-5 days. In two series of the main experiment, the acclimation was over in two and three days when actinomycin D (an inhibitor of DNA-dependent RNA polymerase) was added after various periods of the ciliate incubation at a changed salinity (0, 3, 6, and 24 h). The obtained data indicate that the inhibiting effect of actinomycin D on salinity acclimation of P. calkinsi is manifested only within the first day of the ciliate incubation in an altered environment; by the end of this period their swimming velocity was similar to the control one (acclimation in the absence of the inhibitor). Thus, expression of genes associated with salinity acclimation in paramecium largely completes within the first day, while the acclimation continues for at least 4-5 days. We propose that the acclimation-competent species possess a correspondingly rich genetic program.
通过游泳速度评估，广盐性纤毛虫卡尔金斯草履虫的盐度适应在4 - 5天内完成。在两个系列的主要实验中，当在纤毛虫于变化盐度（0、3、6和24小时）下孵育不同时间段后添加放线菌素D（一种依赖DNA的RNA聚合酶抑制剂）时，适应分别在两天和三天内完成。所获得的数据表明，放线菌素D对卡尔金斯草履虫盐度适应的抑制作用仅在纤毛虫于改变环境中孵育的第一天内表现出来；在此阶段结束时，它们的游泳速度与对照（在无抑制剂情况下的适应）相似。因此，草履虫中与盐度适应相关的基因表达在第一天内基本完成，而适应过程持续至少4 - 5天。我们提出，具有适应能力的物种拥有相应丰富的遗传程序。
