通过苯基琼脂糖凝胶上的疏水相互作用色谱法对里氏木霉纤维素酶进行分级分离。
Fractionation of Trichoderma reesei cellulases by hydrophobic interaction chromatography on phenyl-sepharose.
作者信息
Tomaz Cândida T, Queiroz João A
机构信息
Department of Chemistry, University of Beira Interior, 6201-001 Covilhã, Portugal.
出版信息
Biotechnol Lett. 2004 Feb;26(3):223-7. doi: 10.1023/b:bile.0000013719.92024.04.
Abstract
Trichoderma reesei cellulase complex was fractionated using hydrophobic interaction chromatography with a phenyl-Sepharose column. Using a linear gradient of ammonium sulphate in the eluent buffer, a selective separation of endoglucanases was obtained at 15 degrees C with a four-fold increase in specific activity.
摘要
里氏木霉纤维素酶复合物采用苯基琼脂糖柱通过疏水相互作用色谱法进行分离。在洗脱缓冲液中使用硫酸铵线性梯度，于15℃实现了内切葡聚糖酶的选择性分离，比活性提高了四倍。
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