Levander O A, Morris V C, Ferretti R J
J Nutr. 1977 Mar;107(3):363-72. doi: 10.1093/jn/107.3.363.
The time required for red blood cells (RBC) from vitamin E-deficient lead-poisoned (-E + Pb) rats to pass through polycarbonate filters after incubation in vitro was much greater than that of RBC from vitamin E-supplemented non-poisoned rats. Vitamin E deficiency per se (i.e., in non-poisoned rats) often increased filtration times, but in all such experiments the RBC from -E + Pb groups had even longer filtration times. Administration of lead to rats supplemented with vitamin E had little effect on the filtration rate of RBC. N,N'-diphenyl-p-phenylenediamine (DPPD) prevented the increased filtration times characteristic of RBC from -E + Pb rats, but replacement of the lard in the vitamin E-deficient basal diet by more highly polyunsaturated fats did not exacerbate the increased filtration times of RBC from -E + Pb rats. The increased filtration time of RBC from -E + Pb rats appeared to be related to the extent of RBC lipid peroxidation. Decreasing the pH of the RBC incubation medium from 7.4 to 6.6, an acidity typical of the spleen, markedly increased the filtration times of RBC from -E + Pb rats. Addition of lead in vitro increased filtration times of RBC from both vitamin E-deficient and supplemented non-poisoned rats, but filtration times tended to be longer in the deficient group. These results suggest that vitamin E deficiency and lead toxicity act synergistically to alter the deformability of the RBC thereby rendering it vulnerable to sequestration in the spleen.
在体外孵育后，维生素E缺乏且铅中毒（-E + Pb）大鼠的红细胞（RBC）通过聚碳酸酯滤膜所需的时间，比补充了维生素E且未中毒大鼠的红细胞所需时间长得多。维生素E缺乏本身（即未中毒大鼠）常常会增加过滤时间，但在所有此类实验中，-E + Pb组的红细胞过滤时间更长。给补充了维生素E的大鼠投予铅，对红细胞的过滤速率影响很小。N，N'-二苯基对苯二胺（DPPD）可防止-E + Pb大鼠红细胞出现过滤时间增加的特征，但用多不饱和程度更高的脂肪替代维生素E缺乏基础饮食中的猪油，并不会加剧-E + Pb大鼠红细胞过滤时间的增加。-E + Pb大鼠红细胞过滤时间的增加似乎与红细胞脂质过氧化程度有关。将红细胞孵育培养基的pH从7.4降至6.6（脾脏典型的酸度），会显著增加-E + Pb大鼠红细胞的过滤时间。体外添加铅会增加维生素E缺乏和补充了维生素E的未中毒大鼠红细胞的过滤时间，但缺乏组的过滤时间往往更长。这些结果表明，维生素E缺乏和铅毒性具有协同作用，会改变红细胞的变形能力，从而使其易被脾脏滞留。
