Morgan D L, Dorsey A F, Menzel D B
Fundam Appl Toxicol. 1985 Feb;5(1):137-43. doi: 10.1016/0272-0590(85)90057-0.
Injury from short-term exposure to ozone (O3) was detected by a simple test of red blood cell (RBC) filterability. This test measures changes in the ability of the RBC to deform--as occurs during passage through small capillaries. Male CD-1 mice were exposed to 1.0, 0.7, or 0.3 ppm O3 for 4 hr, and blood samples were obtained by heart puncture. RBCs were suspended in Tris-HCl buffer, pH 7.4, containing 10 mg/dl glucose. After incubation in air for up to 6 hr, the time required for 2 ml RBC suspension to pass through a 3-micron-pore-size polycarbonate filter was determined. A significant increase in the 6-hr filtration time for O3-exposed (1.0 ppm) mice over unexposed mice and a lack of protection by vitamin E were shown. The increases in RBC filtration times for O3-exposed mice appeared to be dose related. Ozone exposure (1.0 ppm) caused a significant increase in the hematocrit of both vitamin E-deficient and -supplemented mice. Vitamin E supplementation appeared to partially prevent this increase in hematocrit. Measurement of lipid peroxidation by the thiobarbituric acid (TBA) test revealed no detectable levels of TBA-reactive material in RBC from O3-exposed mice. These results suggest that measurement of RBC filterability may be feasible as a clinical test for short-term injury from exposure to oxidant gases.
通过一项简单的红细胞（RBC）过滤性测试检测短期暴露于臭氧（O3）造成的损伤。该测试测量红细胞变形能力的变化——这在红细胞通过小毛细血管时会发生。将雄性CD-1小鼠暴露于1.0、0.7或0.3 ppm的O3中4小时，通过心脏穿刺采集血样。将红细胞悬浮于pH 7.4、含有10 mg/dl葡萄糖的Tris-HCl缓冲液中。在空气中孵育长达6小时后，测定2 ml红细胞悬液通过孔径为3微米的聚碳酸酯滤膜所需的时间。结果显示，暴露于O3（1.0 ppm）的小鼠6小时过滤时间比未暴露小鼠显著增加，且维生素E无保护作用。暴露于O3的小鼠红细胞过滤时间的增加似乎与剂量相关。臭氧暴露（1.0 ppm）导致维生素E缺乏和补充维生素E的小鼠的血细胞比容均显著增加。补充维生素E似乎可部分预防这种血细胞比容的增加。通过硫代巴比妥酸（TBA）试验测量脂质过氧化反应，结果显示暴露于O3的小鼠的红细胞中未检测到TBA反应性物质。这些结果表明，测量红细胞过滤性作为暴露于氧化气体所致短期损伤的临床测试可能是可行的。
