Ferencz A, Szántó Z, Kalmár-Nagy K, Horváth O P, Rõth E
Department of Experimental Surgery, Pécs, Hungary.
Transplant Proc. 2004 Mar;36(2):286-8. doi: 10.1016/j.transproceed.2003.12.006.
Ischemic preconditioning (IPC) has been defined as short periods of ischemia with intermittent reperfusion. IPC induces two phases of protection. We sought to investigate the effects of classic and delayed preconditioning on oxidative stress markers prior to autotransplantation. Total orthotopic intestinal autotransplantation was performed on 18 mongrel dogs in three groups: group I (GI, nonpreconditioned), group II (GII, classic preconditioned), and group III (GIII, delayed preconditioned). In GI 3-hour cold preservation in University of Wisconsin solution was followed by 1 hour of reperfusion. In GII before this procedure the intestine was preconditioned by occlusion of the mesenteric artery with four cycles each of 5 minutes of ischemia and 10 minutes of reperfusion (IPC protocol). In GIII on day 1 the animals underwent the IPC protocol, and autotransplantation was performed on day 2. Oxidative stress parameters included malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD) measurements in tissue samples. Our results showed increased lipid peroxidation with decreased GSH level and SOD activity in GI (control: 254.38 +/- 18.32 IU/g; reperfused: 55.01 +/- 26.40 IU/g; P <.05). In GII MDA was slightly elevated, and the GSH concentration was increased markedly. Furthermore, better preservation of SOD activity was observed at the end of the reperfusion. Meanwhile, in GIII GSH was significantly increased, indicating the activation of the endogenous antioxidant protective system (control: 382.13 +/- 24.22 micromol/L per gram; reperfused: 515.25 +/- 26.36 micromol/L per gram; P <.05). Moreover, SOD surpassed the control activity. Our findings confirmed that both forms of preconditioning mitigate the severity of oxidative stress prior to preservation and autotransplantation. Delayed preconditioning is more effective to protect bowel tissue against oxidative injury.
缺血预处理（IPC）被定义为短时间的缺血与间歇性再灌注。IPC诱导两个保护阶段。我们试图研究经典预处理和延迟预处理对自体移植前氧化应激标志物的影响。对18只杂种犬进行全原位小肠自体移植，分为三组：第一组（GI，未预处理）、第二组（GII，经典预处理）和第三组（GIII，延迟预处理）。在GI组中，在威斯康星大学溶液中进行3小时冷保存，随后进行1小时再灌注。在GII组中，在此程序之前，通过肠系膜动脉闭塞进行预处理，缺血5分钟和再灌注10分钟，共四个循环（IPC方案）。在GIII组中，动物在第1天接受IPC方案，并在第2天进行自体移植。氧化应激参数包括组织样本中丙二醛（MDA）、还原型谷胱甘肽（GSH）和超氧化物歧化酶（SOD）的测量。我们的结果显示，GI组脂质过氧化增加，GSH水平和SOD活性降低（对照组：254.38±18.32 IU/g；再灌注组：55.01±26.40 IU/g；P<.05）。在GII组中，MDA略有升高，GSH浓度显著增加。此外，在再灌注结束时观察到SOD活性得到更好的保存。同时，在GIII组中，GSH显著增加，表明内源性抗氧化保护系统被激活（对照组：382.13±24.22微摩尔/升每克；再灌注组：515.25±26.36微摩尔/升每克；P<.05）。此外，SOD超过了对照活性。我们的研究结果证实，两种预处理形式均可减轻保存和自体移植前氧化应激的严重程度。延迟预处理在保护肠组织免受氧化损伤方面更有效。
