Fujisaki S, Tomita R, Park Y J, Kimizuka K, Sugitoh K, Inoue M, Fukuzawa M
First Department of Surgery, Nihon University School of Medicine, Tokyo, Japan.
Transplant Proc. 2004 Mar;36(2):348-9. doi: 10.1016/j.transproceed.2003.12.010.
Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) mediates the homing of lymphocytes to gut-associated lymphoid tissues (GALT). We performed a semiquantative analysis of MAdCAM-1 expression during small bowel graft rejection.
Orthotopic small bowel transplantations (SBT) were performed from BN rats to LEW rats. Isografted animals served as controls. Animals were sacrificed on days 3, 4, 5, 6, and 7 after SBT. Cryostat sections were prepared from grafts, including Peyer's patches (PPs). Indirect immunoperoxidase staining was performed using mAbs against MAdCAM-1. The degree of vascular endothelial staining on high endothelial venules (HEV) in the PPs was graded from 1 (low levels) to 5 (high levels), and in the vessels of the lamina propria from 1 (faint), 2 (low at the base of villi), 3 (low to the middle of villi), 4 (high to the middle of villi), to 5 (high to villus tip).
MAdCAM-1 expression on HEVs in PPs was down-regulated during rejection. In contrast its expression on endothelial cells of vessels in the lamina propria was up-regulated during rejection.
Alteration in MAdCAM-1 expression may be associated with the development of SB graft rejection. The vessels at the base of villi, which are associated with lymphocyte recruitment, may become sites of intense immune reactivity during the early phase of small bowel allograft rejection.
黏膜地址素细胞黏附分子-1（MAdCAM-1）介导淋巴细胞归巢至肠道相关淋巴组织（GALT）。我们对小肠移植排斥反应期间MAdCAM-1的表达进行了半定量分析。
将BN大鼠的小肠原位移植到LEW大鼠体内。同基因移植动物作为对照。在小肠移植术后第3、4、5、6和7天处死动物。从包括派尔集合淋巴结（PPs）在内的移植物制备冰冻切片。使用抗MAdCAM-1的单克隆抗体进行间接免疫过氧化物酶染色。PPs中高内皮微静脉（HEV）上血管内皮染色程度从1级（低水平）到5级（高水平）进行分级，固有层血管的染色程度从1级（微弱）、2级（绒毛底部低水平）、3级（绒毛中部低水平）、4级（绒毛中部高水平）到5级（绒毛顶端高水平）。
排斥反应期间PPs中HEV上的MAdCAM-1表达下调。相反，排斥反应期间固有层血管内皮细胞上的MAdCAM-1表达上调。
MAdCAM-1表达的改变可能与小肠移植排斥反应的发生有关。与淋巴细胞募集相关的绒毛底部血管可能在小肠同种异体移植排斥反应早期成为强烈免疫反应的部位。
