De Faria W, Tryphonopoulos P, Kleiner G, Santiago S, Gandia C, Ruiz P, Tzakis A
Department of Surgery, University of Miami School of Medicine, Miami, FL 33136, USA.
Transplant Proc. 2004 Mar;36(2):375-6. doi: 10.1016/j.transproceed.2004.01.085.
The implantation of fragmented rat intestinal epithelium into the omentum of syngeneic animals results in the formation of a cyst containing neointestine. The purpose of our project was to study the evolution of this neointestine-containing cyst over time. Harvested jejunum and ileum of neonatal DA rats (6 to 8 days old) was digested with collagenase type XI and dispase at room temperature. The resulting organoid units, containing clusters of intestinal epithelium with stem cells were seeded onto a polyglactin polymer mesh (100000 units per mesh). The absorbable mesh was implanted in the omentum or peritoneal wall of an adult syngeneic animal. Animals were sacrificed at weekly intervals to harvest the neointestinal cysts. The lumen of the neointestine cysts was full of mucous while the wall of the cyst was covered by intestinal mucosa. H&E staining of the cyst demonstrated the morphology of intestinal epithelium; PAS staining identified goblet cells. The size of the cyst was maximal between 4 and 8 weeks postimplantation tending to regress thereafter. Neointestinal cysts are a consistent finding after implantation of intestinal epithelium organoids into the omentum or peritoneal wall in the rat model. The cysts reach a maximal size at 4 to 8 weeks postimplantation, tending to regress thereafter.
将碎片化的大鼠肠上皮植入同基因动物的大网膜会导致形成一个含有新肠的囊肿。我们项目的目的是研究这个含新肠囊肿随时间的演变。取新生DA大鼠（6至8日龄）的空肠和回肠，在室温下用XI型胶原酶和 dispase消化。将所得的类器官单位（包含带有干细胞的肠上皮簇）接种到聚乙交酯聚合物网片上（每片网片100000个单位）。将可吸收网片植入成年同基因动物的大网膜或腹膜壁。每隔一周处死动物以收获含新肠的囊肿。新肠囊肿的腔内充满黏液，而囊肿壁被肠黏膜覆盖。囊肿的苏木精-伊红染色显示了肠上皮的形态；过碘酸-希夫染色鉴定出杯状细胞。囊肿大小在植入后4至8周时最大，此后趋于缩小。在大鼠模型中，将肠上皮类器官植入大网膜或腹膜壁后，新肠囊肿是一个常见的发现。囊肿在植入后4至8周达到最大尺寸，此后趋于缩小。
