Cheng Ching-Wen, Lin Kuo-Chin, Pan Fu-Ming, Sinchaikul Supachok, Wong Chi-Huey, Su Wei-Chih, Hsu Ching-Hsiang, Chen Shui-Tein
ALPS Biotech Co. Ltd, Taipei, Taiwan.
Bioorg Med Chem Lett. 2004 Apr 19;14(8):1987-90. doi: 10.1016/j.bmcl.2004.01.084.
A unique peptide sequence of HGGHHG screening from a combinatorial synthetic peptide library showed a good chelating ability to bind a transition metal on a chip better than hexa-His peptide. It was directly conjugated with a His-Tagged proteins onto a chip in a mild aqueous solution and can be used this chip as a high throughput technique for protein array in order to detect and purify the His-Tagged proteins.
从组合合成肽库中筛选出的独特肽序列HGGHHG，在芯片上对过渡金属的螯合能力优于六组氨酸肽。它在温和的水溶液中直接与带His标签的蛋白质偶联到芯片上，并且可以将该芯片用作蛋白质阵列的高通量技术，以检测和纯化带His标签的蛋白质。
