组氨酸突变为酪氨酸的葡萄球菌肠毒素B突变蛋白在C3H/HeJ小鼠中的免疫原性。

Immunogenicity of the histidine-to-tyrosine staphylococcal enterotoxin B mutant protein in C3H/HeJ mice.

作者信息

Savransky Vladimir, Pinelis Dmitriy, Korolev Sergey, Ionin Boris, Fegeding Konstantin

机构信息

Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500, USA.

出版信息

Toxicon. 2004 Mar 15;43(4):433-8. doi: 10.1016/j.toxicon.2004.02.013.

DOI:10.1016/j.toxicon.2004.02.013

PMID:15051407

Abstract

Staphylococcal enterotoxin B (SEB) is a common cause of food poisoning and toxic shock. A safe and effective vaccine is needed to protect against the superantigenic effects of this toxin. We previously constructed and produced an apparently nontoxic SEB mutant having four histidine-to-tyrosine substitutions in positions 12, 32, 105, and 121. In the present study, we found that this H1.2.3.4 SEB mutant had low toxicity, was able to induce high levels of specific IgG antibodies, and protected mice in both the actinomycin D-primed and intranasal SEB intoxication model systems, despite the absence of detectable specific IgM and IgA antibodies. We propose further development of the H1.2.3.4 recombinant protein as a potential anti-SEB vaccine candidate.

摘要

葡萄球菌肠毒素B（SEB）是食物中毒和中毒性休克的常见病因。需要一种安全有效的疫苗来预防这种毒素的超抗原效应。我们之前构建并生产了一种明显无毒的SEB突变体，其在第12、32、105和121位有四个组氨酸到酪氨酸的替换。在本研究中，我们发现这种H1.2.3.4 SEB突变体毒性低，能够诱导高水平的特异性IgG抗体，并且在放线菌素D预处理和鼻内SEB中毒模型系统中均能保护小鼠，尽管未检测到特异性IgM和IgA抗体。我们建议进一步开发H1.2.3.4重组蛋白作为潜在的抗SEB疫苗候选物。