Jiang Wen, Wang Jin-Cun, Zhang Zhi, Sheerin Aaron H, Zhang Xia
Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Brain Res. 2004 May 1;1006(2):248-52. doi: 10.1016/j.brainres.2004.02.005.
In this study we examined the unknown issue of whether seizure-induced newborn hippocampal neurons in freely moving adult rats are able to respond to pathophysiological stimuli in the same way as their neighboring neurons do. Three days after pentylenetrazol (PTZ)-induced generalized seizures, rats received 5-bromodeoxyuridine (BrdU) injections to label dividing cells, followed 4 weeks later by the second PTZ injection to induce second episode of generalized seizures. We observed that the first episode of PTZ-induced seizures resulted in a significant increase in the number of newborn neurons in the adult hippocampal dentate gyrus. In comparison with vehicle-injected control rats that exhibited no Fos immunoreactivity and mild glutamic acid decarboxylase 67 (GAD67) expression in the dentate granule cells, rats killed 2-6 h following the second PTZ injection showed intensive Fos and GAD67 expression in virtually all granule cells with or without BrdU double-labeling. These findings provide important evidence indicating that seizure-induced newborn neurons in freely moving adult rats are able to respond to pathophysiological stimuli in the same way as neighboring neurons do.
在本研究中，我们探讨了一个未知问题，即自由活动的成年大鼠中癫痫发作诱导产生的新生海马神经元是否能够像其相邻神经元一样对病理生理刺激作出反应。在戊四氮（PTZ）诱导的全身性癫痫发作三天后，给大鼠注射5-溴脱氧尿苷（BrdU）以标记分裂细胞，4周后进行第二次PTZ注射以诱导第二次全身性癫痫发作。我们观察到，第一次PTZ诱导的癫痫发作导致成年海马齿状回中新生神经元数量显著增加。与未注射药物的对照大鼠相比，对照大鼠在齿状颗粒细胞中未表现出Fos免疫反应性且谷氨酸脱羧酶67（GAD67）表达轻微，而在第二次PTZ注射后2 - 6小时处死的大鼠中，几乎所有颗粒细胞中无论有无BrdU双标记均显示出强烈的Fos和GAD67表达。这些发现提供了重要证据，表明自由活动的成年大鼠中癫痫发作诱导产生的新生神经元能够像相邻神经元一样对病理生理刺激作出反应。
