Nahar Pradip, Naqvi Azmi, Basir Seemi Farhat
Institute of Genomics and Integrative Biology (CSIR), Delhi University Campus, Mall Road, Delhi 110007, India.
Anal Biochem. 2004 Apr 15;327(2):162-4. doi: 10.1016/j.ab.2003.11.030.
Polystyrene, polypropylene, and polyethylene surfaces were activated by exposing 1-fluoro-2-nitro-4-azidobenzene coated surface to sunlight. Sunlight intensity of 26,300 lux was found optimum beyond which no appreciable increase in activation was observed. Five-minutes sunlight exposure gave better activated surface than 5 min 365-nm UV light exposure. The efficacy of sunlight-mediated activated surfaces was demonstrated by covalently immobilizing proteins onto them. Horseradish peroxidase when immobilized onto the sunlight-activated surfaces showed more than twofold increase in immobilization than the surface without activation. Thus, sunlight being a versatile, eco-friendly, and clean energy source can be a potential alternative for activation of inert surface for covalent attachment of biomolecule such as protein, DNA, or carbohydrate.
通过将涂有1-氟-2-硝基-4-叠氮苯的表面暴露在阳光下，对聚苯乙烯、聚丙烯和聚乙烯表面进行活化。发现26,300勒克斯的阳光强度是最佳的，超过此强度，活化效果无明显增加。暴露于阳光下5分钟比暴露于365纳米紫外线下5分钟能产生更好的活化表面。通过将蛋白质共价固定在这些表面上，证明了阳光介导的活化表面的功效。当辣根过氧化物酶固定在阳光活化的表面上时，其固定量比未活化的表面增加了两倍多。因此，阳光作为一种多功能、环保且清洁能源，可以成为活化惰性表面以共价连接蛋白质、DNA或碳水化合物等生物分子的潜在替代方法。
