Dublineau I, Dudoignon N, Monti P, Combes O, Wysocki J, Grison S, Baudelin C, Griffiths N M, Scanff P
Institut de Radioprotection et de Sûreté Nucléaire, Direction de la RadioProtection de l'Homme, Service de Radiobiologie et d'Epidemiologie, IRSN, BP 17, F-92262 CEDEX Fontenay-aux-Roses, France.
Can J Physiol Pharmacol. 2004 Feb;82(2):103-13. doi: 10.1139/y03-130.
The aim of this study was to assess the potential of gastrointestinal peptide plasma levels as biomarkers of radiation-induced digestive tract damage. To this end, plasma levels of substance P, GRP, motilin, PYY, somatostatin-28, gastrin, and neurotensin were followed for up to 5 days in pigs after a 16-Gy whole-body X-irradiation, completed by a histopathological study performed at 5 days. Each peptide gave a specific response to irradiation. The plasma levels of GRP and substance P were not modified by irradiation exposure; neither were those of motilin and PYY. Concerning gastrin, a 2-3-fold increase of plasma concentration was observed in pig, which presented the most important histological alterations of the stomach. The plasma levels of somatostatin, unchanged from 1 to 4 days after irradiation, was also increased by 130% at 5 days. In contrast, a diminution of neurotensin plasma levels was noted, firstly at 1 day (-88%), and from 3 days after exposure (-50%). The present study suggested that changes in gastrin and neurotensin plasma levels were associated with structural alterations of the stomach and ileum, respectively, indicating that they may be relevant biological indicators of radiation-induced digestive damage to these segments.
本研究的目的是评估胃肠肽血浆水平作为辐射诱导消化道损伤生物标志物的潜力。为此，在16 Gy全身X射线照射后的猪中，对P物质、胃泌素释放肽（GRP）、胃动素、肽YY（PYY）、生长抑素-28、胃泌素和神经降压素的血浆水平进行了长达5天的跟踪，并在第5天进行了组织病理学研究。每种肽对辐射都有特定的反应。GRP和P物质的血浆水平未因辐射暴露而改变；胃动素和PYY的血浆水平也未改变。关于胃泌素，在猪中观察到血浆浓度增加了2至3倍，其胃呈现出最重要的组织学改变。生长抑素的血浆水平在照射后1至4天未发生变化，但在第5天也增加了130%。相反，神经降压素的血浆水平降低，首先在第1天降低了88%，在暴露后3天降低了50%。本研究表明，胃泌素和神经降压素血浆水平的变化分别与胃和回肠的结构改变相关，表明它们可能是辐射诱导这些节段消化损伤的相关生物学指标。
