Fischer B, Benzina S, Ganansia-Leymarie V, Denis J M, Bergerat J P, Dufour P, Gueulette J, Bischoff P
Laboratoire de Cancérologie Expérimentale et Radiobiologie, EA-3430, IRCAD, Hôpitaux Universitaires, 1 place de l'Hôpital, F-67091 Strasbourg CEDEX, France.
Can J Physiol Pharmacol. 2004 Feb;82(2):140-5. doi: 10.1139/y04-007.
The utilization of high linear energy transfer (LET) radiations, such as fast neutrons or carbon ions (hadrontherapy), offers promising perspectives in radiotherapy. While it is well known that by combining radiotherapy and chemotherapy, important therapeutic advantages can be obtained to cure cancer, there have been, so far, very few investigations on the effects of treatments combining an irradiation with high-LET particles and cancer drugs. The present study was therefore undertaken to examine the effects of exposure to 65 MeV fast neutrons combined with cisplatin in a murine T cell lymphoma (RDM4) in vitro. The cells were irradiated at doses ranging from 2 to 8 Gy without or with addition of cisplatin shortly before the irradiation, at concentrations between 0.3 and 12.5 micro M. These treatments were applied concomitantly. Proliferation and apoptosis were assessed at different time intervals thereafter. The combination of irradiation with cisplatin was found to be more cytotoxic than either treatment alone. Furthermore, the cytotoxicity induced by this cotreatment resulted not only from apoptosis but also from other forms of cell death.
高传能线密度（LET）辐射的应用，如快中子或碳离子（强子治疗），在放射治疗中展现出了广阔的前景。虽然众所周知，将放射治疗与化疗相结合可以获得重要的治疗优势以治愈癌症，但迄今为止，关于高LET粒子照射与抗癌药物联合治疗效果的研究却非常少。因此，本研究旨在体外检测65 MeV快中子照射联合顺铂对小鼠T细胞淋巴瘤（RDM4）的影响。细胞在2至8 Gy的剂量范围内进行照射，在照射前不久，添加浓度为0.3至12.5微摩尔的顺铂或不添加顺铂。这些处理同时进行。此后在不同时间间隔评估细胞增殖和凋亡情况。结果发现，照射与顺铂联合使用比单独任何一种治疗的细胞毒性更大。此外，这种联合治疗诱导的细胞毒性不仅源于凋亡，还源于其他形式的细胞死亡。
