Kayaselcuk Fazilet, Serin Ender, Gumurdulu Yüksel, Ozer Birol, Tuncer Ilhan, Boyacioglu Sedat
Department of Pathology, Adana Teaching and Medical Research Center, Baskent University, 39 sok. Yuregir/Adana, Turkey.
World J Gastroenterol. 2004 Apr 1;10(7):1056-8. doi: 10.3748/wjg.v10.i7.1056.
Our aims were to determine the normal limits of subepithelial basement membrane (SEBM) thickness in order to more accurately diagnose collagenous colitis in the population from southern Turkey and to investigate into links between SEBM thickness and age, and sex.
The study included 100 patients (mean age 50.0+/-13.3 years; male, 34; female, 66) with miscellaneous gastrointestinal symptoms, and normal colonic mucosal appearance in colonoscopic evaluation. Biopsies were taken from five different regions of the colon. SEBM was measured with a calibrated eyepiece on specimens prepared with specific stains for collagen. Intensity of inflammatory cells was graded semiquantitatively. Differences in SEBM thickness among the different colon regions, and relationships between SEBM thickness and age, sex, and density of inflammatory cells were statistically evaluated.
The cecum and rectum showed the largest amounts of infiltrate. None of the specimens showed histologic findings of collagenous colitis. The SEBM thicknesses measured for each case ranged from 3-20 microm. The biggest thickness was observed in rectal mucosa (median value: 10 microm). Cecum and ascending colon showed similar SEBM thickness (median value: 5 microm). SEBM thickness was not correlated with patient age or sex, but was positively correlated with the intensity of inflammatory cells in each colon segment.
In this patient group from southern Turkey, SEBM was thickest in the rectum. Our results indicate that, in this population, SEBM thickness is not correlated with age or sex, but is positively correlated with severity of inflammation. The findings also support the concept that measuring SEBM thickness at one segment in the colon is inadequate and may be misleading.
我们的目的是确定上皮下基底膜（SEBM）厚度的正常范围，以便更准确地诊断土耳其南部人群中的胶原性结肠炎，并研究SEBM厚度与年龄及性别的关系。
该研究纳入了100例有各种胃肠道症状且结肠镜检查显示结肠黏膜外观正常的患者（平均年龄50.0±13.3岁；男性34例，女性66例）。从结肠的五个不同区域取活检组织。使用校准目镜在经胶原特异性染色制备的标本上测量SEBM。对炎症细胞的强度进行半定量分级。对不同结肠区域之间SEBM厚度的差异以及SEBM厚度与年龄、性别和炎症细胞密度之间的关系进行统计学评估。
盲肠和直肠的浸润量最大。所有标本均未显示胶原性结肠炎的组织学表现。每个病例测量的SEBM厚度范围为3至20微米。直肠黏膜的厚度最大（中位数：10微米）。盲肠和升结肠的SEBM厚度相似（中位数：5微米）。SEBM厚度与患者年龄或性别无关，但与每个结肠段炎症细胞的强度呈正相关。
在来自土耳其南部的该患者组中，直肠的SEBM最厚。我们的结果表明，在该人群中，SEBM厚度与年龄或性别无关，但与炎症严重程度呈正相关。这些发现还支持这样的观点，即在结肠的一个节段测量SEBM厚度是不够的，可能会产生误导。
