氧化型低密度脂蛋白对人肾小球系膜细胞A类清道夫受体表达的影响

[Effects of oxidized low-density lipoprotein on expression of type A scavenger receptor in human mesangial cells].

作者信息

Ye Wen-ling, Li Xue-wang, Xu Cai-min, Duan Lin, Li Yan, Jin Jing

机构信息

Department of Nephrology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2004 Feb;26(1):34-7.

PMID:15052771

Abstract

OBJECTIVE

To explore the regulational effect of oxidized low-density lipoprotein (Ox-LDL) on expression of type A scavenger receptor (SR-A) in human mesangial cells (HMC).

METHODS

HMC line (HMCL) with high expression of SR-A (HMCL-SRA) was established after stable transfection of expressive vector with cDNA encoding SR-A. Uptake of Ox-LDL by HMCL was evaluated using Oil Red "O" staining. SR-A mRNA expression was examined using reverse transcription-polymerase chain reaction (RT-PCR).

RESULTS

More uptake of Ox-LDL was observed in the HMCL-SRA than that in the untransfected HMCL. Ox-LDL could induce SR-A mRNA expression in HMC in a dose-dependent manner, and reached a peak level after 24 h of stimulation. After 24 h of stimulation with Ox-LDL at the dose of 10, 50 and 100 micrograms/ml, SR-A mRNA expression was up-regulated to 1.35, 1.83 and 2.30-fold of controls, respectively. However, LDL had no effect on the expression of SR-A.

CONCLUSIONS

It suggests that SR-A be a major binding receptor to uptake Ox-LDL in HMC. Ox-LDL may promote the progression of chronic renal diseases through up-regulation of SR-A.

摘要

目的

探讨氧化型低密度脂蛋白（Ox-LDL）对人肾小球系膜细胞（HMC）中A类清道夫受体（SR-A）表达的调节作用。

方法

用编码SR-A的cDNA表达载体稳定转染后，建立SR-A高表达的HMC系（HMCL）（HMCL-SRA）。采用油红“O”染色评估HMCL对Ox-LDL的摄取。用逆转录-聚合酶链反应（RT-PCR）检测SR-A mRNA表达。

结果

与未转染的HMCL相比，HMCL-SRA对Ox-LDL的摄取更多。Ox-LDL能以剂量依赖的方式诱导HMC中SR-A mRNA表达，并在刺激24小时后达到峰值水平。用10、50和100微克/毫升的Ox-LDL刺激24小时后，SR-A mRNA表达分别上调至对照的1.35、1.83和2.30倍。然而，低密度脂蛋白（LDL）对SR-A的表达没有影响。