Fernández P E, Barbeito C G, Portiansky E L, Gimeno E J
Institute of Pathology, Faculty of Veterinary Sciences, National University of La Plata, PO Box 296, 1900 La Plata, Argentina.
Vet Rec. 2004 Mar 6;154(10):298-303. doi: 10.1136/vr.154.10.298.
Hyperplasia and squamous metaplasia of the prostatic epithelium are conditions induced by oestrogens. Diethylstilbestrol (DES) has been banned from cattle used for beef production because of the health risks. The potential use of molecular markers for the detection of illegal oestrogen administration was evaluated by taking samples of prostatic tissue from control bullocks, bullocks which had been treated with oestrogens, and bullocks sacrificed 21 and 90 days after a single dose of DES. The expression of the glycoconjugates was examined by lectinhistochemistry and the lectin binding pattern was characterised in epithelium and connective tissue. In the animals sacrificed after 21 days there was an increase in the binding of one lectin (JAC) and there was an increase in the binding of one of the other lectins (DBA) in the animals sacrificed after 90 days. An increase in SWGA lectin staining was observed in the bullocks that had probably been treated with oestrogen and in the animals sacrificed 90 days after the inoculation with DES. There were also differences between the binding of SWGA in the control bullocks and the other groups.
前列腺上皮的增生和鳞状化生是由雌激素诱导的病症。己烯雌酚（DES）由于健康风险已被禁止用于肉牛生产。通过从对照公牛、接受过雌激素处理的公牛以及单次注射DES后21天和90天宰杀的公牛采集前列腺组织样本，评估了分子标记物用于检测非法施用雌激素的潜在用途。通过凝集素组织化学检查糖缀合物的表达，并在上皮和结缔组织中表征凝集素结合模式。在21天后宰杀的动物中，一种凝集素（JAC）的结合增加，而在90天后宰杀的动物中，另一种凝集素（DBA）的结合增加。在可能接受过雌激素处理的公牛以及接种DES后90天宰杀的动物中，观察到SWGA凝集素染色增加。对照公牛与其他组之间的SWGA结合也存在差异。
