Shin Sung Jae, Chang Yung-Fu, Huang Cathy, Zhu Jiaqian, Huang Lester, Yoo Han Sang, Shin Kwang-Soon, Stehman Susan, Shin Sang J, Torres Alfonso
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
J Vet Diagn Invest. 2004 Mar;16(2):116-20. doi: 10.1177/104063870401600204.
A polymerase chain reaction (PCR) assay for confirmation of Mycobacterium avium subsp. paratuberculosis was developed using the primer set derived from ISMav2. The PCR product was 494 base pairs (bp) and could be digested with ClaI, which produced 311- and 183-bp fragments. No amplification of 494-bp DNA fragment was detected from DNA of other Mycobacterium spp., including Mycobacterium avium complex, other bacteria, including Escherichia coli, Pseudomonas aeruginosa, Actinobacillus pleuropneumoniae, Leptospira interrogans serovar pomona, Corynebacterium pseudotuberculosis, Salmonella typhimurium, Borrelia burgdorferi, and Staphylococcus aureus, and the Scedosporium sp. This PCR assay could detect 5-8 genome equivalents.
利用源自ISMav2的引物对，开发了一种用于确认副结核分枝杆菌鸟分枝杆菌亚种的聚合酶链反应（PCR）检测方法。PCR产物为494个碱基对（bp），可用ClaI消化，产生311 bp和183 bp的片段。从其他分枝杆菌属的DNA中未检测到494 bp DNA片段的扩增，包括鸟分枝杆菌复合群、其他细菌，如大肠杆菌、铜绿假单胞菌、胸膜肺炎放线杆菌、波摩那钩端螺旋体、伪结核棒状杆菌、鼠伤寒沙门氏菌、伯氏疏螺旋体和金黄色葡萄球菌，以及头孢霉属。这种PCR检测方法可检测5至8个基因组当量。
