Ritchie M R, Morton M S, Thompson A M, Deighton N, Blake A, Cummings J H, Steel C M
Bute Medical School, University of St. Andrews, Fife, Scotland.
Eur J Clin Nutr. 2004 Sep;58(9):1286-9. doi: 10.1038/sj.ejcn.1601963.
To study the variation in genistein + daidzein intake over a 6-month period and test the reliability of 24 h urinary isoflavones as a biomarker of exposure over time.
Dietary genistein + daidzein intake was assessed at various time points throughout six months in 15 healthy subjects. Group 1 (n=8) followed nonsupplemented diets and Group 2 (n=7) took a 35 mg/d isoflavone supplement for 3 months and each subject provided a 24 h urine collection, validated with para-aminobenzoic acid, during weeks 7, 15 and 19. Urine was analysed for genistein and daidzein using LC-MS.
Isoflavone intake in Groups 1 and 2 ranged from 0.00 to 1.1 mg/d and 0.1 to 53.1 mg/d, respectively. Urine excretion for both groups ranged from 0.20 to 9.56 mg/d. The relationship between 24 h excretion and isoflavone intake is y=0.44 x +/- 0.03(standard deviation) + 1.57; r=0.89, P<0.001.
The 24 h urinary isoflavones can be used as biomarkers of isoflavone exposure over time.
研究6个月期间染料木黄酮+黄豆苷元摄入量的变化，并测试24小时尿异黄酮作为随时间变化的暴露生物标志物的可靠性。
在6个月内的不同时间点评估了15名健康受试者的膳食染料木黄酮+黄豆苷元摄入量。第1组（n = 8）遵循非补充饮食，第2组（n = 7）服用35毫克/天的异黄酮补充剂，为期3个月，每位受试者在第7、15和19周提供一份用对氨基苯甲酸验证的24小时尿液样本。使用液相色谱-质谱法分析尿液中的染料木黄酮和黄豆苷元。
第1组和第2组的异黄酮摄入量分别为0.00至1.1毫克/天和0.1至53.
