一种在电泳凝胶中检测催产素酶活性的新技术。

A new technique for detecting oxytocinase activity in electrophoresis gels.

作者信息

Roy A C, Saha N, Tan S M, Kamarul F Z, Ratnam S S

机构信息

Department of Obstetrics and Gynaecology, National University of Singapore.

出版信息

Electrophoresis. 1992 Jun;13(6):396-7. doi: 10.1002/elps.1150130181.

DOI:10.1002/elps.1150130181

PMID:1505501

Abstract

A sensitive staining method for the detection of oxytocinase (EC 3.4.11.3) activity in electrophoresis gels has been described. The method is based on the enzymatic release of p-nitroaniline (PNA) from two specific synthetic oxytocinase substrates, S-benzyl-L-cysteine-p-nitroanilide (BCN) and L-leucine-p-nitroanilide (LN), respectively. The PNA is then diazotized with sodium nitrite and subsequently coupled to a chromogen, N-(1-naphthyl)-ethylenediamine dihydrochloride (NED) to produce a deep pink/magenta colored azo-dye at the site of oxytocinase activity.

摘要

本文描述了一种用于检测电泳凝胶中催产素酶（EC 3.4.11.3）活性的灵敏染色方法。该方法基于分别从两种特定的合成催产素酶底物S-苄基-L-半胱氨酸对硝基苯胺（BCN）和L-亮氨酸对硝基苯胺（LN）中酶促释放对硝基苯胺（PNA）。然后，PNA用亚硝酸钠重氮化，随后与显色剂N-（1-萘基）-乙二胺二盐酸盐（NED）偶联，在催产素酶活性位点产生深粉色/品红色的偶氮染料。