U1小核RNA的变体组装成剪接体复合物。

Variants of U1 small nuclear RNA assemble into spliceosomal complexes.

作者信息

Pereira-Simon S, Sierra-Montes J M, Ayesh K, Martinez L, Socorro A, Herrera R J

机构信息

Department of Biological Sciences, Florida International University, Miami, FL 33199, USA.

出版信息

Insect Mol Biol. 2004 Apr;13(2):189-94. doi: 10.1111/j.0962-1075.2004.00476.x.

DOI:10.1111/j.0962-1075.2004.00476.x

PMID:15056366

Abstract

In this study, the existence of additional U1 snRNA variants in the posterior silk gland of the Bombyx mori Nistari strain from India was investigated. Three new U1 variants were detected. One of the new isoforms (U1 SG1) was found to be preferentially assembled into high molecular weight spliceosomal complexes in comparison with the total cellular lysate RNA control. Structural and nucleotide differences were examined in these new isoforms and compared with the previously reported U1 variants. Free energy (Delta G) values for the entire U1 snRNA secondary structures as well as the individual stem/loops (I, II, III and IV) domains of the isoforms were estimated to determine their structural stability.

摘要

在本研究中，对来自印度的家蚕Nistari品系后部丝腺中额外的U1 snRNA变体的存在情况进行了调查。检测到三个新的U1变体。与总细胞裂解物RNA对照相比，发现其中一种新的异构体（U1 SG1）优先组装成高分子量剪接体复合物。对这些新异构体的结构和核苷酸差异进行了检查，并与先前报道的U1变体进行了比较。估计了异构体的整个U1 snRNA二级结构以及各个茎/环（I、II、III和IV）结构域的自由能（ΔG）值，以确定其结构稳定性。