Calappi E, Masserini M, Schiavo G, Montecucco C, Tettamanti G
Department of Medical Chemistry and Biochemistry, University of Milano, Italy.
FEBS Lett. 1992 Sep 7;309(2):107-10. doi: 10.1016/0014-5793(92)81074-v.
The interaction of Tetanus toxin with phospholipid vesicles containing gangliosides (GD1a, GD1b or GT1b) or phosphatidic acid has been investigated at neutral or acidic pH. Change in the thermotropic properties of the vesicles occurred only after addition of the toxin at acidic pH, and led to surface binding or membrane insertion of the protein, dependent on the physical state of the membrane. Most remarkably, toxin addition at acidic pH to dipalmitoyl-phosphatidylcholine vesicles containing GT1b ganglioside, caused formation of ganglioside microdomains on the vesicle surface.
已在中性或酸性pH条件下研究了破伤风毒素与含有神经节苷脂（GD1a、GD1b或GT1b）或磷脂酸的磷脂囊泡之间的相互作用。仅在酸性pH下添加毒素后，囊泡的热致性质才会发生变化，并导致蛋白质的表面结合或膜插入，这取决于膜的物理状态。最显著的是，在酸性pH下将毒素添加到含有GT1b神经节苷脂的二棕榈酰磷脂酰胆碱囊泡中，会导致囊泡表面形成神经节苷脂微结构域。
