Nagai M, Endoh M, Burns D L, Nakase Y
Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
FEMS Microbiol Lett. 1992 Jun 1;72(2):133-7. doi: 10.1016/0378-1097(92)90518-s.
The effect of Bordetella heat-labile toxin (HLT) on cultured vascular or tracheal smooth cells labeled with [14C]arachidonic acid for 3 h was examined. At 37 degrees C, in the presence of Ca2+, HLT induced the release of radioactive substances from the cells in a dose-dependent manner but HLT had no effect on release of radioactivity from cells at 0 degrees C or in the absence of Ca2+. After cells were exposed to HLT, a 2-h lag period occurred before release of radioactivity was detected. The substances released from cells by HLT were identified as arachidonic acid and phosphatidylcholine.
研究了百日咳杆菌不耐热毒素（HLT）对用[14C]花生四烯酸标记3小时的培养血管或气管平滑肌细胞的影响。在37℃、有Ca2+存在的情况下，HLT以剂量依赖的方式诱导细胞释放放射性物质，但HLT在0℃或无Ca2+时对细胞放射性释放没有影响。细胞暴露于HLT后，在检测到放射性释放之前有2小时的延迟期。HLT从细胞中释放的物质被鉴定为花生四烯酸和磷脂酰胆碱。
