高效液相色谱法测定血浆和组织中的利福平
High-performance liquid-chromatographic determination of rifampicin in plasma and tissues.
作者信息
Calleja Ignacio, Blanco-Príeto María J, Ruz Noelia, Renedo María Jesús, Dios-Viéitez María Carmen
机构信息
Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Navarra, C/Irunlarrea 1, 31080 Pamplona, Spain.
出版信息
J Chromatogr A. 2004 Mar 26;1031(1-2):289-94. doi: 10.1016/j.chroma.2003.12.041.
A HPLC-UV method has been developed for assaying rifampicin in plasma and liver. The assay involved a liquid-liquid extraction procedure with dichloromethane-pentane (1:1). An Ultrabase-C18 column and a simple mobile phase consisting of a water (pH 2.27)-acetonitrile (40:60, v/v) mixture were used. The flow-rate was 1 ml/min and the effluent was monitored at 333 nm. Results from the HPLC analyses showed that the assay method is linear in the ranges 0.1-1 and 1-50 microg/ml for plasma, and 0.6-40 microg/g for liver. Intra- and inter-day R.S.D. were below 15% for all the sample types. Recoveries averaged 83 and 95% for plasma and liver, respectively. The method is being successfully applied to determine rifampicin in plasma and liver samples taken during pharmacokinetic studies in rats.
已开发出一种用于测定血浆和肝脏中利福平的高效液相色谱 - 紫外检测法。该检测方法包括用二氯甲烷 - 戊烷（1:1）进行液 - 液萃取步骤。使用了Ultrabase - C18柱和由水（pH 2.27） - 乙腈（40:60，v/v）混合物组成的简单流动相。流速为1 ml/min，流出物在333 nm处进行监测。高效液相色谱分析结果表明，该检测方法在血浆中0.1 - 1和1 - 50 μg/ml以及肝脏中0.6 - 40 μg/g的范围内呈线性。所有样品类型的日内和日间相对标准偏差均低于15%。血浆和肝脏的回收率分别平均为83%和95%。该方法已成功应用于测定大鼠药代动力学研究期间采集的血浆和肝脏样品中的利福平。
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