利用随机扩增多态性聚合酶链反应（RAPD-PCR）和温度梯度凝胶电泳（TTGE）评估格拉纳·帕达诺奶酪乳清培养物的生物多样性。

Use of RAPD-PCR and TTGE for the evaluation of biodiversity of whey cultures for Grana Padano cheese.

作者信息

Andrighetto C, Marcazzan G, Lombardi A

机构信息

Veneto Agricoltura, Istituto per la Qualità e le Tecnologie Agroalimentari, Thiene (VI), Italy.

出版信息

Lett Appl Microbiol. 2004;38(5):400-5. doi: 10.1111/j.1472-765X.2004.01504.x.

DOI:10.1111/j.1472-765X.2004.01504.x

PMID:15059211

Abstract

AIMS

This work was carried out in order to evaluate the microbial diversity of whey cultures collected from different Grana Padano cheese plants in Veneto region (north-east Italy) by means of RAPD-PCR and Temporal Temperature Gradient Gel Electrophoresis (TTGE) analysis.

METHODS AND RESULTS

Lactobacillus helveticus was the dominant species among isolated thermophilic lactobacilli. RAPD-PCR with primers M13 and D8635 resulted a suitable method for typing Lact. helveticus at strain level. Thirteen different Lact. helveticus biotypes were detected in the seven whey cultures studied with one biotype present in all the whey cultures. Besides Lact. helveticus, Lact. delbrueckii subsp. lactis was the main microbial species detected by TTGE.

CONCLUSIONS

RAPD-PCR resulted very useful in studying Lact. helveticus biodiversity; furthermore, TTGE analysis allowed to detect the dominant thermophilic microflora characteristic of Grana Padano cheese whey cultures.

IMPACT OF THE STUDY

By the combined used of RAPD-PCR and TTGE it could be possible to follow the behaviour in strain or species composition of whey cultures during time.

摘要

目的

开展本研究是为了通过随机扩增多态性DNA聚合酶链反应（RAPD-PCR）和时间温度梯度凝胶电泳（TTGE）分析，评估从意大利东北部威尼托地区不同格拉纳·帕达诺奶酪工厂收集的乳清培养物中的微生物多样性。

方法与结果

瑞士乳杆菌是分离出的嗜热乳杆菌中的优势种。用引物M13和D8635进行RAPD-PCR是在菌株水平上对瑞士乳杆菌进行分型的合适方法。在所研究的7种乳清培养物中检测到13种不同的瑞士乳杆菌生物型，所有乳清培养物中都存在1种生物型。除瑞士乳杆菌外，德氏乳杆菌保加利亚亚种是通过TTGE检测到的主要微生物种类。