沙门氏菌属和单核细胞增生李斯特菌在塑料表面形成生物膜。
Biofilm formation by Salmonella spp. and Listeria monocytogenes on plastic surface.
作者信息
Stepanović S, Cirković I, Ranin L, Svabić-Vlahović M
机构信息
Institute of Microbiology and Immunology, School of Medicine, Belgrade, Serbia.
出版信息
Lett Appl Microbiol. 2004;38(5):428-32. doi: 10.1111/j.1472-765X.2004.01513.x.
AIMS
To investigate the biofilm formation by 122 Salmonella spp. and 48 Listeria monocytogenes strains on a plastic surface.
METHODS
Quantification of biofilm formation was performed in brain heart infusion (BHI), trypcase soya broth (TSB), meat broth (MB) and 1/20 diluted trypcase soya broth (1/20-TSB) in plastic microtitre plates.
RESULTS
All tested Salmonella spp. and L. monocytogenes strains produced biofilm in a suitable medium. However, the quantities of biofilm produced by Salmonella spp. were greater than those produced by tested L. monocytogenes strains. The nutrient content of the medium significantly influenced the quantity of produced biofilm. Diluted TSB was the most effective in promoting biofilm production by Salmonella spp., followed by TSB, while the least quantity of biofilm was formed in BHI and MB. L. monocytogenes produced the highest quantities of biofilm in BHI, followed by TSA, then MB, and the least quantities of biofilm were produced in 1/20-TSB.
CONCLUSIONS
Salmonella spp. produces more biofilm in nutrient-poor medium, while L. monocytogenes produce more biofilm in nutrient-rich medium.
目的
研究122株沙门氏菌属菌株和48株单核细胞增生李斯特菌在塑料表面形成生物膜的情况。
方法
在塑料微量滴定板中，于脑心浸液（BHI）、胰蛋白胨大豆肉汤（TSB）、肉汁肉汤（MB）和1/20稀释胰蛋白胨大豆肉汤（1/20 - TSB）中进行生物膜形成的定量分析。
结果
所有测试的沙门氏菌属菌株和单核细胞增生李斯特菌菌株在合适的培养基中均能产生生物膜。然而，沙门氏菌属产生的生物膜量大于测试的单核细胞增生李斯特菌菌株产生的生物膜量。培养基的营养成分显著影响生物膜的产生量。稀释的TSB对促进沙门氏菌属生物膜的产生最为有效，其次是TSB，而在BHI和MB中形成的生物膜量最少。单核细胞增生李斯特菌在BHI中产生的生物膜量最高，其次是TSA，然后是MB，在1/20 - TSB中产生的生物膜量最少。
结论
沙门氏菌属在营养贫乏的培养基中产生更多生物膜，而单核细胞增生李斯特菌在营养丰富的培养基中产生更多生物膜。
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